Thyrotropin-releasing hormone and its receptor in glia

• Published in: Glia 2001-03, Vol.33 (4), p.267-276
• Main Author: Fernández-Agulló, Teresa
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Monoclonal; Astrocytes - chemistry; Astrocytes - cytology; astroglia; Biological and medical sciences; Blotting, Western; Cells, Cultured; Cerebral Cortex - chemistry; Cerebral Cortex - cytology; Fundamental and applied biological sciences. Psychology; Immunohistochemistry; Isolated neuron and nerve. Neuroglia; Male; Rats; Rats, Wistar; receptor; Receptors, Thyrotropin-Releasing Hormone - analysis; Receptors, Thyrotropin-Releasing Hormone - immunology; Spinal Cord - chemistry; Spinal Cord - cytology; Thyrotropin-Releasing Hormone - analysis; Thyrotropin-Releasing Hormone - immunology; TRH; Vertebrates: nervous system and sense organs
• ISSN: 0894-1491; 1098-1136
• DOI: 10.1002/1098-1136(20010315)33:4<267::AID-GLIA1025>3.0.CO;2-1

The presence of thyrotropin‐releasing hormone (Thyroliberin, TRH) and its receptor (TRH‐R) in frozen coronal sections of the adult rat spinal cord and neonatal rat astroglial cultures was investigated by means of immunocytochemistry and Western blot using polyclonal antibodies generated against the hormone and monoclonal antibodies originated against discrete sequences of the type 1 rat TRH receptor (TRH‐R1). TRH‐R1 and TRH are present both in astroglial cells from adult rats and in cultured cells from newborn animals. The localization of TRH and TRH‐R1 in nonneuronal cells in the central nervous system may reflect that some of the neurotrophic actions of TRH upon the central nervous system are mediated by glial cells. GLIA 33:267–276, 2001. © 2001 Wiley‐Liss, Inc.