Phenotypic Distinction and Functional Characterization of Pro-B Cells in Adult Mouse Bone Marrow

A lymphoid-committed progenitor population was isolated from mouse bone marrow based on the cell surface phenotype Thy-1.1(neg)Sca-1(pos)c-Kit(low)Lin(neg). These cells were CD43(pos)CD24(pos) on isolation and proliferated in response to the cytokine combination of steel factor, IL-7, and Flt3 ligan...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of immunology (1950) 2001-03, Vol.166 (5), p.3042-3051
Main Authors: Mojica, Mariluz P, Perry, S. Scott, Searles, A. Elena, Elenitoba-Johnson, Kojo S. J, Pierce, L. Jeanne, Wiesmann, Anne, Slayton, William B, Spangrude, Gerald J
Format: Article
Language:English
Subjects:
Aging - genetics
Aging - immunology
Animals
Antigens, Differentiation, B-Lymphocyte - biosynthesis
B-Lymphocyte Subsets - cytology
B-Lymphocyte Subsets - immunology
B-Lymphocyte Subsets - metabolism
Bone Marrow Cells - cytology
Bone Marrow Cells - immunology
Bone Marrow Cells - metabolism
CD24 antigen
CD43 antigen
Cell Differentiation - genetics
Cell Differentiation - immunology
Cell Lineage - genetics
Cell Lineage - immunology
Cell Separation
Cells, Cultured
Gene Expression Regulation, Developmental - immunology
Hyaluronan Receptors
Immunophenotyping
Membrane Glycoproteins
Mice
Mice, Inbred AKR
Mice, Inbred C57BL
Mitochondrial Proteins
Myeloid Cells - cytology
Myeloid Cells - immunology
Myeloid Cells - metabolism
Proto-Oncogene Proteins c-kit - biosynthesis
Receptors, Complement - biosynthesis
Stem Cells - cytology
Stem Cells - immunology
Stem Cells - metabolism
Thy-1 Antigens - biosynthesis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A lymphoid-committed progenitor population was isolated from mouse bone marrow based on the cell surface phenotype Thy-1.1(neg)Sca-1(pos)c-Kit(low)Lin(neg). These cells were CD43(pos)CD24(pos) on isolation and proliferated in response to the cytokine combination of steel factor, IL-7, and Flt3 ligand. Lymphoid-committed progenitors could be segregated into more primitive and more differentiated subsets based on expression of AA4.1. The more differentiated subset generated only B lymphoid cells in 92% of total colonies assayed, lacked T lineage potential, and expressed Pax5. These studies have therefore defined and isolated a B lymphoid-committed progenitor population at a developmental stage corresponding to the initial expression of CD45R.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.166.5.3042