On-site diagnosis of H. pylori infection by urine

• Published in: Diagnostic microbiology and infectious disease 2001-02, Vol.39 (2), p.95-97
• Main Authors: Miwa, Hiroto, Akamatsu, Suguru, Tachikawa, Tetsuya, Sogabe, Takayuki, Ohtaka, Keiichi, Nagahara, Akihito, Sugiyama, Yuriko, Sato, Nobuhiro
• Format: Article
• Language: English
• Subjects: 13C urea breath test; Antigens, Bacterial - blood; Bacterial diseases; Bacterial diseases of the digestive system and abdomen; Bacterial diseases of the urinary system; Biological and medical sciences; Breath Tests; Helicobacter Infections - diagnosis; Helicobacter Infections - microbiology; Helicobacter pylori - isolation & purification; Human bacterial diseases; Humans; Infectious diseases; Medical sciences; non-invasive test; rapid urine kit; rapid whole blood test; Reagent Kits, Diagnostic; Sensitivity and Specificity; Urea - metabolism; Urine - microbiology
• ISSN: 0732-8893; 1879-0070
• DOI: 10.1016/S0732-8893(01)00205-X

We have recently developed an on-site diagnostic kit for H. pylori infection using urine (utilizing immunochromatographic method employing a nitrocellulose membrane coated by extracted H. pylori antigen). Accordingly, we investigated its usefulness in 155 consecutive dyspeptic patients using the 13C urea breath test as a gold standard and further compared its performance with two commercially available rapid diagnostic kits that use whole blood (Helisal Rapid Blood™, and ImmunoCard H. pylori™). As the results, the urine based on-site diagnostic kit provided 95.9% sensitivity and 87.9% specificity with 92.9% accuracy, which were comparable or even better than that of both rapid whole blood tests, suggesting its usefulness in screening of H. pylori infection.