Differential anthracycline sensitivity in two related human colon carcinoma cell lines expressing similar levels of P-glycoprotein

• Published in: Cancer letters 2001-04, Vol.165 (1), p.111-116
• Main Authors: Boquete, Alejandra L, Vargas Roig, Laura, López, Gustavo A, Gude, Rajiv, Binda, M.Mercedes, González, Alejandro D, Ciocca, Daniel R, Bonfil, R.Daniel
• Format: Article
• Language: English
• Subjects: 4′-Epidoxorubicin; Anthracyclines; Antibiotics, Antineoplastic - metabolism; Antibiotics, Antineoplastic - pharmacology; ATP Binding Cassette Transporter, Subfamily B, Member 1 - metabolism; Biological and medical sciences; Blotting, Western; Cell Survival; Colon carcinoma; Colonic Neoplasms; Drug Resistance, Neoplasm; Drug Screening Assays, Antitumor; Epirubicin - metabolism; Epirubicin - pharmacology; Gastroenterology. Liver. Pancreas. Abdomen; Heat shock proteins; HSP27 Heat-Shock Proteins; HSP70 Heat-Shock Proteins - analysis; Humans; Immunohistochemistry; Medical sciences; Multidrug resistance; Neoplasm Proteins - analysis; P-glycoprotein; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Tumor Cells, Cultured; Tumors
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/S0304-3835(01)00410-4

Chemosensitivity of the human colon carcinoma HCT-15 cell line to 4′-epidoxorubicin proved to be 100-fold higher than that of its variant HCT-15 EDR. Confocal scanning microscopy showed significant less drug accumulation in HCT-15 EDR. A 2-fold increase in hsp27 expression was found in HCT-15 EDR, with no alteration in hsp70. The expression of the drug exporter Pgp was similar in both cell lines, despite the lower drug accumulation shown by HCT-15 EDR in respect to HCT-15. Other molecules implicated in the acquisition of enhanced chemoresistance or a more active Pgp variant present in HCT-15 EDR, could explain the phenomenon.