Isolation and characterization of the exopolysaccharide produced by Streptococcus thermophilus SFi20

This paper reports isolation, structural characterization and some physico-chemical properties in aqueous solution of the exopolysaccharide (EPS) produced by Streptococcus thermophilus strain SFi20. The yield of the purified EPS was found to be reproducible and close to the average value of 143 mg/l...

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Bibliographic Details
Published in:International journal of biological macromolecules 2001-03, Vol.28 (3), p.219-226
Main Authors: Navarini, Luciano, Abatangelo, Anna, Bertocchi, Claudia, Conti, Elena, Bosco, Marco, Picotti, Fabrizio
Format: Article
Language:English
Subjects:
Carbohydrate Conformation
Carbohydrate Sequence
Characterization
Circular Dichroism
Exopolysaccharide
Isolation
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Molecular Weight
Polysaccharides, Bacterial - chemistry
Polysaccharides, Bacterial - isolation & purification
Rheology
Streptococcus - chemistry
Streptococcus thermophilus
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Summary:This paper reports isolation, structural characterization and some physico-chemical properties in aqueous solution of the exopolysaccharide (EPS) produced by Streptococcus thermophilus strain SFi20. The yield of the purified EPS was found to be reproducible and close to the average value of 143 mg/l. The chemical structure, previously suggested, has been confirmed on the basis of NMR data. Viscometric, chiro-optical and rheological measurements have been carried out with the aim of characterizing the conformational state of the polysaccharide in aqueous solution. All the data reported indicate that the EPS does not undergo a cooperative conformational transition under the investigated experimental conditions. Furthermore, the viscosity data and the viscoelastic behaviour suggest that the polymer is rather flexible and adopts a random coil conformation in aqueous solution.
ISSN:0141-8130
1879-0003
DOI:10.1016/S0141-8130(01)00118-0