Accumulation of barley stripe mosaic virus is significantly reduced in transgenic wheat plants expressing a bacterial ribonuclease

An rnc70 gene encoding a mutant bacterial ribonuclease III (RNase III) was introduced into wheat (Triticum aestivum cv. Bobwhite) by microprojectile bombardment. T1, T2, and T3 plants regenerated from three transgenic callus lines were challenged with barley stripe mosaic virus. Plants expressing RN...

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Bibliographic Details
Published in:Transgenic research 2001, Vol.10 (1), p.13-19
Main Authors: LINGYU ZHANG, FRENCH, Roy, LANGENBERG, Willem G, MITRA, Amitava
Format: Article
Language:English
Subjects:
Bacteria
Barley stripe mosaic virus
Biological and medical sciences
Biotechnology
Blotting, Western
Cloning, Molecular
DNA Primers - chemistry
Endoribonucleases - genetics
Endoribonucleases - metabolism
Fundamental and applied biological sciences. Psychology
Gene Expression
Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control
Genetic engineering
Genetic technics
Genetics
Hordeum - virology
Methods. Procedures. Technologies
Mosaic Viruses - genetics
Mosaic Viruses - physiology
Mutation
Phytopathology. Animal pests. Plant and forest protection
Plant viruses and viroids
Plants, Genetically Modified - enzymology
Plants, Genetically Modified - genetics
Polymerase Chain Reaction
Ribonuclease III
RNA, Viral - genetics
RNA, Viral - metabolism
rnc70 gene
Transformation, Genetic
Transgenic animals and transgenic plants
Transgenic plants
Triticum - enzymology
Triticum - virology
Triticum aestivum
Virus Replication
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Summary:An rnc70 gene encoding a mutant bacterial ribonuclease III (RNase III) was introduced into wheat (Triticum aestivum cv. Bobwhite) by microprojectile bombardment. T1, T2, and T3 plants regenerated from three transgenic callus lines were challenged with barley stripe mosaic virus. Plants expressing RNase III exhibited a high level of resistance to the virus infection. This resistance was evidenced by the absence of virus symptoms and reduced accumulation of virions in these plants. The result demonstrates that this pathogen-targeted resistance strategy can be effectively employed in conferring resistance to viral diseases of cereal crops.
ISSN:0962-8819
1573-9368
DOI:10.1023/A:1008931706679