MMP-28, a new human matrix metalloproteinase with an unusual cysteine-switch sequence is widely expressed in tumors

We report the discovery, cloning, and characterization of a novel human matrix metalloproteinase (MMP-28) cDNA gene. The deduced 520-amino-acid sequence of MMP-28 includes a signal peptide, a prodomain with an unusual cysteine-switch PRCGVTD motif followed by the furin cleavage RRKKR site, a catalyt...

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Bibliographic Details
Published in:Gene 2001-03, Vol.265 (1-2), p.87-93
Main Authors: Marchenko, G N, Strongin, A Y
Format: Article
Language:English
Subjects:
Alternative Splicing
Amino Acid Motifs
Amino Acid Sequence
chromosome 17
Chromosome Mapping
Chromosomes, Human, Pair 17 - genetics
Cloning, Molecular
Cysteine - genetics
DNA, Complementary - chemistry
DNA, Complementary - genetics
DNA, Complementary - metabolism
Evolution, Molecular
Exons
Female
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Neoplastic
Genes - genetics
Humans
Introns
Male
Matrix metalloproteinase
Matrix Metalloproteinases - chemistry
Matrix Metalloproteinases - genetics
Matrix Metalloproteinases, Secreted
MMP-28 gene
Molecular Sequence Data
Neoplasms - enzymology
Neoplasms - genetics
Neoplasms - pathology
Phylogeny
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Tissue Distribution
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Summary:We report the discovery, cloning, and characterization of a novel human matrix metalloproteinase (MMP-28) cDNA gene. The deduced 520-amino-acid sequence of MMP-28 includes a signal peptide, a prodomain with an unusual cysteine-switch PRCGVTD motif followed by the furin cleavage RRKKR site, a catalytic domain, a hinge-region and a hemopexin-like domain. On the basis of their structural characteristics, MMP-28 belongs to the MMP-19 subfamily. The genomic MMP-28 gene uniquely mapped to chromosome 17q11.2 includes eight exons and seven introns. The broad range of expression in carcinomas as well as normal adult and fetal tissues suggests an important functional role for MMP-28.
ISSN:0378-1119
DOI:10.1016/S0378-1119(01)00360-2