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Granulocyte macrophage-colony-stimulating factor mRNA is stabilized in airway eosinophils and peripheral blood eosinophils activated by TNF-alpha plus fibronectin
Airway eosinophils show prolonged in vitro survival compared with peripheral blood eosinophils (PBEos). Recent studies have shown that autocrine production and release of GM-CSF is responsible for enhanced survival, but the mechanisms controlling cytokine production remain obscure. We compared GM-CS...
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| Published in: | The Journal of immunology (1950) 2001-04, Vol.166 (7), p.4658-4663 |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c402t-69138a123272829eee2c0670825ce92a83b53f9b78d200e5189686966bb20f983 |
| container_end_page | 4663 |
| container_issue | 7 |
| container_start_page | 4658 |
| container_title | The Journal of immunology (1950) |
| container_volume | 166 |
| creator | Esnault, S Malter, J S |
| description | Airway eosinophils show prolonged in vitro survival compared with peripheral blood eosinophils (PBEos). Recent studies have shown that autocrine production and release of GM-CSF is responsible for enhanced survival, but the mechanisms controlling cytokine production remain obscure. We compared GM-CSF mRNA decay in eosinophils from bronchoalveolar lavage (BALEos) after allergen challenge or from PBEos. BALEos showed prolonged survival in vitro (60% at 4 days) and expressed GM-CSF mRNA. The enhanced survival of BALEos was 75% inhibited at 6 days by neutralizing anti-GM-CSF Ab. Based on transfection studies, GM-CSF mRNA was 2.5 times more stable in BALEos than in control PBEos. Treatment of PBEos with fibronectin and TNF-alpha increased their in vitro survival, GM-CSF mRNA expression, and GM-CSF mRNA stability to a comparable level as seen in BALEos. These data suggest that TNF-alpha plus fibronectin may increase eosinophil survival in vivo by controlling GM-CSF production at a posttranscriptional level. |
| doi_str_mv | 10.4049/jimmunol.166.7.4658 |
| format | article |
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Recent studies have shown that autocrine production and release of GM-CSF is responsible for enhanced survival, but the mechanisms controlling cytokine production remain obscure. We compared GM-CSF mRNA decay in eosinophils from bronchoalveolar lavage (BALEos) after allergen challenge or from PBEos. BALEos showed prolonged survival in vitro (60% at 4 days) and expressed GM-CSF mRNA. The enhanced survival of BALEos was 75% inhibited at 6 days by neutralizing anti-GM-CSF Ab. Based on transfection studies, GM-CSF mRNA was 2.5 times more stable in BALEos than in control PBEos. Treatment of PBEos with fibronectin and TNF-alpha increased their in vitro survival, GM-CSF mRNA expression, and GM-CSF mRNA stability to a comparable level as seen in BALEos. 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Recent studies have shown that autocrine production and release of GM-CSF is responsible for enhanced survival, but the mechanisms controlling cytokine production remain obscure. We compared GM-CSF mRNA decay in eosinophils from bronchoalveolar lavage (BALEos) after allergen challenge or from PBEos. BALEos showed prolonged survival in vitro (60% at 4 days) and expressed GM-CSF mRNA. The enhanced survival of BALEos was 75% inhibited at 6 days by neutralizing anti-GM-CSF Ab. Based on transfection studies, GM-CSF mRNA was 2.5 times more stable in BALEos than in control PBEos. Treatment of PBEos with fibronectin and TNF-alpha increased their in vitro survival, GM-CSF mRNA expression, and GM-CSF mRNA stability to a comparable level as seen in BALEos. These data suggest that TNF-alpha plus fibronectin may increase eosinophil survival in vivo by controlling GM-CSF production at a posttranscriptional level.</description><subject>Bronchi - cytology</subject><subject>Bronchi - immunology</subject><subject>Bronchi - metabolism</subject><subject>Bronchoalveolar Lavage Fluid - chemistry</subject><subject>Bronchoalveolar Lavage Fluid - cytology</subject><subject>Cell Movement - immunology</subject><subject>Cell Survival - drug effects</subject><subject>Cell Survival - immunology</subject><subject>Cells, Cultured</subject><subject>Drug Combinations</subject><subject>Eosinophils - drug effects</subject><subject>Eosinophils - immunology</subject><subject>Eosinophils - metabolism</subject><subject>Fibronectins - pharmacology</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - blood</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - genetics</subject><subject>Granulocyte-Macrophage Colony-Stimulating Factor - metabolism</subject><subject>Humans</subject><subject>RNA, Messenger - blood</subject><subject>RNA, Messenger - metabolism</subject><subject>Tumor Necrosis Factor-alpha - pharmacology</subject><subject>Up-Regulation - immunology</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNqFkc1qFjEUQIMo9rPtEwiSlbv5TDIz-VmWYqtQWih1HZLMnTYlk4zJjGV8HJ_UlH4irlzdzbnnwj0Ivadk35FOfXr007TGFPaU873Yd7yXr9CO9j1pOCf8NdoRwlhDBRdH6F0pj4QQTlj3Fh1RyvpOsH6Hfl1mE9eQ3LYAnozLaX4w99C4FFLcmrL4aQ1m8fEej8YtKePp9voM-4LLYqwP_icM2EdsfH4yG4ZUfKwKHwo2ccAzZD8_QDYB25DS8C_gFv_DLFVgN3x3fdGYUI_jOawFj97mFKES8QS9GU0ocHqYx-jbxee78y_N1c3l1_Ozq8Z1hC0NV7SVhrKWCSaZAgDmCBdEst6BYka2tm9HZYUcGCHQU6m45IpzaxkZlWyP0ccX75zT9xXKoidfHIRgIqS1aMGV7Fj7f5AKSWsAXsH2BaxvLSXDqOfsJ5M3TYl-bqj_NNS1oRb6uWHd-nDQr3aC4e_OIVr7G_AhnYU</recordid><startdate>20010401</startdate><enddate>20010401</enddate><creator>Esnault, S</creator><creator>Malter, J S</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20010401</creationdate><title>Granulocyte macrophage-colony-stimulating factor mRNA is stabilized in airway eosinophils and peripheral blood eosinophils activated by TNF-alpha plus fibronectin</title><author>Esnault, S ; Malter, J S</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c402t-69138a123272829eee2c0670825ce92a83b53f9b78d200e5189686966bb20f983</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Bronchi - cytology</topic><topic>Bronchi - immunology</topic><topic>Bronchi - metabolism</topic><topic>Bronchoalveolar Lavage Fluid - chemistry</topic><topic>Bronchoalveolar Lavage Fluid - cytology</topic><topic>Cell Movement - immunology</topic><topic>Cell Survival - drug effects</topic><topic>Cell Survival - immunology</topic><topic>Cells, Cultured</topic><topic>Drug Combinations</topic><topic>Eosinophils - drug effects</topic><topic>Eosinophils - immunology</topic><topic>Eosinophils - metabolism</topic><topic>Fibronectins - pharmacology</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - blood</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - genetics</topic><topic>Granulocyte-Macrophage Colony-Stimulating Factor - metabolism</topic><topic>Humans</topic><topic>RNA, Messenger - blood</topic><topic>RNA, Messenger - metabolism</topic><topic>Tumor Necrosis Factor-alpha - pharmacology</topic><topic>Up-Regulation - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Esnault, S</creatorcontrib><creatorcontrib>Malter, J S</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Esnault, S</au><au>Malter, J S</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Granulocyte macrophage-colony-stimulating factor mRNA is stabilized in airway eosinophils and peripheral blood eosinophils activated by TNF-alpha plus fibronectin</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>2001-04-01</date><risdate>2001</risdate><volume>166</volume><issue>7</issue><spage>4658</spage><epage>4663</epage><pages>4658-4663</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>Airway eosinophils show prolonged in vitro survival compared with peripheral blood eosinophils (PBEos). Recent studies have shown that autocrine production and release of GM-CSF is responsible for enhanced survival, but the mechanisms controlling cytokine production remain obscure. We compared GM-CSF mRNA decay in eosinophils from bronchoalveolar lavage (BALEos) after allergen challenge or from PBEos. BALEos showed prolonged survival in vitro (60% at 4 days) and expressed GM-CSF mRNA. The enhanced survival of BALEos was 75% inhibited at 6 days by neutralizing anti-GM-CSF Ab. Based on transfection studies, GM-CSF mRNA was 2.5 times more stable in BALEos than in control PBEos. Treatment of PBEos with fibronectin and TNF-alpha increased their in vitro survival, GM-CSF mRNA expression, and GM-CSF mRNA stability to a comparable level as seen in BALEos. These data suggest that TNF-alpha plus fibronectin may increase eosinophil survival in vivo by controlling GM-CSF production at a posttranscriptional level.</abstract><cop>United States</cop><pmid>11254725</pmid><doi>10.4049/jimmunol.166.7.4658</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of immunology (1950), 2001-04, Vol.166 (7), p.4658-4663 |
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| language | eng |
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| source | EZB-FREE-00999 freely available EZB journals |
| subjects | Bronchi - cytology Bronchi - immunology Bronchi - metabolism Bronchoalveolar Lavage Fluid - chemistry Bronchoalveolar Lavage Fluid - cytology Cell Movement - immunology Cell Survival - drug effects Cell Survival - immunology Cells, Cultured Drug Combinations Eosinophils - drug effects Eosinophils - immunology Eosinophils - metabolism Fibronectins - pharmacology Granulocyte-Macrophage Colony-Stimulating Factor - blood Granulocyte-Macrophage Colony-Stimulating Factor - genetics Granulocyte-Macrophage Colony-Stimulating Factor - metabolism Humans RNA, Messenger - blood RNA, Messenger - metabolism Tumor Necrosis Factor-alpha - pharmacology Up-Regulation - immunology |
| title | Granulocyte macrophage-colony-stimulating factor mRNA is stabilized in airway eosinophils and peripheral blood eosinophils activated by TNF-alpha plus fibronectin |
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