Restricted growth of ent− and tonB mutants of Salmonella enterica serovar Typhi in human Mono Mac 6 monocytic cells

• Published in: FEMS microbiology letters 2001-03, Vol.196 (1), p.7-11
• Main Authors: Gorbacheva, Victoria Y., Faundez, Gustavo, Godfrey, Henry P., Cabello, Felipe C.
• Format: Article
• Language: English
• Subjects: Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacteriology; Biological and medical sciences; Biological Transport; Cell Line; DNA Transposable Elements; Enterobactin - metabolism; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Genetics; Humans; In vivo growth restriction; Iron; Iron - metabolism; Iron uptake mutant; Macrophages; Membrane Proteins - genetics; Membrane Proteins - metabolism; Microbiology; Mono Mac 6 monocytic cell; Monocytes; Monocytes - microbiology; Mutation; Pathogenesis; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Pathogens; Salmonella; Salmonella enterica; Salmonella enterica Typhi; Salmonella typhi - genetics; Salmonella typhi - growth & development; Salmonella typhi - pathogenicity; TonB; Virulence
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.2001.tb10532.x

Abstract Monocytes and macrophages are an important host defense in humans infected with Salmonella enterica serovar Typhi. Bacterial ability to survive in these cells is therefore a crucial virulence characteristic of this pathogen. In this study, we demonstrate that growth of a Salmonella enterica serovar Typhi enterochelin synthesis mutant and a tonB mutant in the human monocyte cell line Mono Mac 6 is restricted compared to that of the parental wild-type Ty2 strain. These results suggest that enterochelin- and TonB-mediated iron uptake plays a role in S. enterica serovar Typhi pathogenesis, and also suggest that mutations in iron uptake may attenuate S. enterica serovar Typhi strains for human beings.