Hepatitis C virus genotyping versus serotyping in Egyptian patients

The RNA genome of hepatitis C virus (HCV) displays extensive sequence variation. In this study, serotyping and genotyping techniques were applied to assess this variability by comparing the performance of the serotyping assay with a panel of well-characterized HCV strains isolated from chronic activ...

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Bibliographic Details
Published in:Infection 2001-01, Vol.29 (1), p.24-26
Main Authors: ZEKRI, A.-R. N, BAHNASSY, A. A, RAMADAN, A. S, EL-BASSUONI, M, BADRAN, A, MADWAR, M. A
Format: Article
Language:English
Subjects:
Biological and medical sciences
Egypt
Genotype
Hepacivirus - classification
Hepacivirus - genetics
Hepacivirus - isolation & purification
Hepatitis
Hepatitis C
Hepatitis C - immunology
Hepatitis C - virology
Hepatitis C virus
Human viral diseases
Humans
Infectious diseases
Medical sciences
Reverse Transcriptase Polymerase Chain Reaction
Sensitivity and Specificity
Serotyping - methods
Tropical medicine
Viral diseases
Viral hepatitis
Viremia - immunology
Viremia - virology
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Summary:The RNA genome of hepatitis C virus (HCV) displays extensive sequence variation. In this study, serotyping and genotyping techniques were applied to assess this variability by comparing the performance of the serotyping assay with a panel of well-characterized HCV strains isolated from chronic active hepatitis (CAH) patients. 60 serum samples from CAH patients were analyzed. All isolates were genotyped by a line probe assay and the results of genotyping and serotyping were evaluated. The overall sensitivity of the serotyping and genotyping techniques was 81.16% with a concordance of 73.3%. Type 4 was detected in 73.3% of cases and it was highly heterogeneous. Type 4 HCV is the most prevalent type in Egyptian CAH patients and there is a high concordance between the results of serotyping and genotyping techniques.
ISSN:0300-8126
1439-0973
DOI:10.1007/s15010-001-0010-8