Vaccination with Her-2/neu DNA or protein subunits protects against growth of a Her-2/neu-expressing murine tumor

The present study utilizes an in vivo murine tumor expressing human Her-2/neu to evaluate potential Her-2/neu vaccines consisting of either full length or various subunits of Her-2/neu delivered in either protein or plasmid DNA form. Our results demonstrate that protective immunity against Her-2/neu...

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Bibliographic Details
Published in:Vaccine 2001-03, Vol.19 (17), p.2598-2606
Main Authors: Foy, Teresa M, Bannink, Jeannette, Sutherland, Robert A, McNeill, Patricia D, Moulton, Garner G, Smith, John, Cheever, Martin A, Grabstein, Kenneth
Format: Article
Language:English
Subjects:
Animals
Antineoplastic agents
Biological and medical sciences
Cancer Vaccines - genetics
Cancer Vaccines - pharmacology
CD4 antigen
CD4-Positive T-Lymphocytes - immunology
CD8 antigen
Female
Genes, erbB-2
HER2 protein
Immunotherapy
Medical sciences
Mice
Mice, Inbred C57BL
Neoplasms, Experimental - immunology
Neoplasms, Experimental - pathology
Neoplasms, Experimental - prevention & control
neu protein
Pharmacology. Drug treatments
Protein Subunits
Receptor, ErbB-2 - chemistry
Receptor, ErbB-2 - genetics
Receptor, ErbB-2 - immunology
Thymoma - immunology
Thymoma - pathology
Thymoma - therapy
Thymus Neoplasms - immunology
Thymus Neoplasms - pathology
Thymus Neoplasms - therapy
Tumor Cells, Cultured
Vaccines, DNA - genetics
Vaccines, DNA - pharmacology
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Summary:The present study utilizes an in vivo murine tumor expressing human Her-2/neu to evaluate potential Her-2/neu vaccines consisting of either full length or various subunits of Her-2/neu delivered in either protein or plasmid DNA form. Our results demonstrate that protective immunity against Her-2/neu-expressing tumor challenge can be achieved by vaccination with plasmid DNA encoding either full length or subunits of Her-2/neu. Partial protective immunity was also observed following vaccination with the intracellular domain (ICD), but not extracellular domain (ECD), protein subunit of Her-2/neu. The mechanism of protection elicited by plasmid DNA vaccination appeared to be exclusively CD4 dependent, whereas the protection observed with ICD protein vaccination required both CD4 and CD8 T cells.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(00)00493-X