Towards a protein interaction map of potyviruses: protein interaction matrixes of two potyviruses based on the yeast two-hybrid system

A map for the interactions of the major proteins from Potato virus A (PVA) and Pea seed-borne mosaic virus (PSbMV) (members of the genus Potyvirus, family Potyviridae) was generated using the yeast two-hybrid system (YTHS). Interactions were readily detected with five PVA protein combinations (HC-HC...

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Bibliographic Details
Published in:Journal of general virology 2001-04, Vol.82 (4), p.935-939
Main Authors: Guo, D, Rajamaki, M.L, Saarma, M, Valkonen, J.P.T
Format: Article
Language:English
Subjects:
AHC protein
Hybridization, Genetic
NIa protein
NIb protein
Pea seed-borne mosaic virus
Potato virus A
Potyvirus - chemistry
protein-protein interactions
two hybrid system techniques
viral proteins
Viral Proteins - chemistry
Viral Proteins - physiology
Virus Replication
viruses
VPg protein
Yeasts - genetics
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Summary:A map for the interactions of the major proteins from Potato virus A (PVA) and Pea seed-borne mosaic virus (PSbMV) (members of the genus Potyvirus, family Potyviridae) was generated using the yeast two-hybrid system (YTHS). Interactions were readily detected with five PVA protein combinations (HC-HC, HC-Cl, VPg-VPg, Nla-Nlb and CP-CP) and weak but reproducible interactions were detected for seven additional combinations (P1-Cl, P3-Nlb, NlaPro-Nlb, VPg-Nla, VPg-NlaPro, NlaPro-Nla and Nla-Nla). In PSbMV, readily detectable interactions were found in five protein combinations (HC-HC, VPg-VPg, VPg-Nla, Nla-Nla and Nla-Nlb) and weaker but reproducible interactions were detected for three additional combinations (P3-Nla, Nla-NlaPro and CP-CP). The self-interactions of HC, VPg, Nla and CP and the interactions of VPg-Nla, Nla-NIaPro and Nla-Nlb were, therefore, common for the two potyviruses. The multiple protein interactions revealed in this study shed light on the co-ordinated functions of potyviral proteins involved in virus movement and replication.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-82-4-935