High-energy phosphate metabolism and creatine kinase in failing hearts: A new porcine model

• Published in: Circulation (New York, N.Y.) N.Y.), 2001-03, Vol.103 (11), p.1570-1576
• Main Authors: YUN YE, GUANGRONG GONG, OCHIAI, Koichi, JINGBO LIU, JIANYI ZHANG
• Format: Article
• Language: English
• Subjects: Adenosine Triphosphate - metabolism; Animals; Aortic Valve Stenosis - complications; Binding, Competitive; Biological and medical sciences; Biomarkers - analysis; Biopsy; Blood Pressure; Cardiology. Vascular system; Chromium - metabolism; Creatine Kinase - metabolism; Disease Models, Animal; Energy Metabolism; Heart; Heart Failure - enzymology; Heart Failure - metabolism; Heart Failure - physiopathology; Heart failure, cardiogenic pulmonary edema, cardiac enlargement; Heart Rate; Hypertrophy, Left Ventricular - enzymology; Hypertrophy, Left Ventricular - metabolism; Hypertrophy, Left Ventricular - physiopathology; Kinetics; Magnetic Resonance Imaging; Medical sciences; Phosphates - metabolism; Phosphorus Radioisotopes; Protein Isoforms - metabolism; Regional Blood Flow; Swine; Ventricular Dysfunction, Left - physiopathology
• ISSN: 0009-7322; 1524-4539
• DOI: 10.1161/01.CIR.103.11.1570

This study aimed to create a pig model of heart failure secondary to severe aortic stenosis and to examine the relationship between the alterations in myocardial high-energy phosphate (HEP) metabolism and protein expression of creatine kinase (CK) isoforms. Sixteen pigs with left ventricular hypertrophy (LVH) secondary to ascending aortic banding and 10 normal pigs (N) were studied. Myocardial protein levels of CK isoforms (Western blot), HEP levels, and CK kinetics ((31)P MR spectroscopy) were measured under basal conditions. Nine of the 16 animals with LVH developed congestive heart failure (CHF), as evidenced by ascites (100 to 2000 mL). LV weight/body weight ratio (g/kg) was 2.18+/-0.15 in N hearts, 3.04+/-0.14 in hearts with LVH (P