HLA class II associations with Type 1 diabetes mellitus: a multivariate approach

• Published in: Tissue antigens 2001-02, Vol.57 (2), p.144-150
• Main Authors: Schipper, R.F., Koeleman, B.P.C., Bruining, G.J., Schreuder, G.M.tH, Verduijn, W., De Vries, R.R.P., Roep, B.O.
• Format: Article
• Language: English
• Subjects: Diabetes Mellitus, Type 1 - genetics; Diabetes Mellitus, Type 1 - immunology; Genetic Predisposition to Disease; Haplotypes; histocompatibility antigen HLA; Histocompatibility Antigens Class II - genetics; HLA class II; Humans; insulin-dependent diabetes mellitus; multi variate analysis; Multivariate Analysis; Odds Ratio; Phenotype
• ISSN: 0001-2815; 1399-0039
• DOI: 10.1034/j.1399-0039.2001.057002144.x

: The association of HLA class II phenotype with the development of insulin‐dependent (Type 1) diabetes mellitus (IDDM) is well established but the contribution of the various HLA‐DR and ‐DQ alleles and haplotypes to disease predisposition is not fully understood. We have determined haplotype and genotype odds ratios, and further employed multivariate tree analysis to explore the contribution of individual HLA‐DRDQ haplotypes to the genetic risk for developing IDDM in the Dutch population. Next to haplotype and genotype odds ratios, multivariate tree analysis techniques provide overall risk calculations for each modeled parameter, and offer insight in the interaction of the model parameters via tree‐shaped reports, in which subsequent stratifications on the data can easily be followed. We compared 206 Dutch IDDM patients with 840 serologically typed random healthy unrelated Dutch Caucasoid controls. The multivariate tree analysis showed that the HLA‐DR7DQ9 and DR15DQ6 haplotype were strongly associated with disease protection (OR=0.04, P=0.0003, and OR=0.07, P=