Synthesis and Characterization of Fluorescent Cobalamin (CobalaFluor) Derivatives for Imaging

Fluorescent derivatives of cobalamin have been prepared by linking fluorophores to cobalamin through a propylamide spacer. Fluorescein, naphthofluorescein, and Oregon Green derivatives have been prepared in good yield by reaction of the fluorophore NHS-ester with β-(3-aminopropyl)cobalamin to form f...

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Bibliographic Details
Published in:Organic letters 2001-03, Vol.3 (6), p.799-801
Main Authors: Smeltzer, Claudia C, Cannon, Michelle J, Pinson, Patrick R, Munger, John D, West, Frederick G, Grissom, Charles B
Format: Article
Language:English
Subjects:
Boron Compounds
Fluoresceins - chemical synthesis
Fluoresceins - chemistry
Fluorescent Dyes - chemical synthesis
Fluorescent Dyes - chemistry
Humans
Indicators and Reagents
Molecular Conformation
Molecular Structure
Neoplasms - diagnosis
Receptors, Cell Surface - analysis
Spectrophotometry
Vitamin B 12 - chemical synthesis
Vitamin B 12 - chemistry
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Summary:Fluorescent derivatives of cobalamin have been prepared by linking fluorophores to cobalamin through a propylamide spacer. Fluorescein, naphthofluorescein, and Oregon Green derivatives have been prepared in good yield by reaction of the fluorophore NHS-ester with β-(3-aminopropyl)cobalamin to form fluorescent cobalamin conjugates (CobalaFluors) that are potentially suitable for the in vitro and in vivo imaging of transcobalamin receptors on cancer cells.
ISSN:1523-7060
1523-7052
DOI:10.1021/ol006825v