Significant fetal-maternal hemorrhage after termination of pregnancy: Implications for development of fetal cell microchimerism

Objective: Recent reports that an association exists between fetal cell microchimerism and autoimmune disease has increased interest in the postpartum persistence of fetal cells. The purpose of this study was to determine, by means of quantitative polymerase chain reaction amplification, whether a s...

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Bibliographic Details
Published in:American journal of obstetrics and gynecology 2001-03, Vol.184 (4), p.703-706
Main Authors: Bianchi, Diana W., Farina, Antonio, Weber, William, Delli-Bovi, Laurent C., DeRiso, Matthew, Williams, John M., Klinger, Katherine W.
Format: Article
Language:English
Subjects:
Abortion, Induced
Biological and medical sciences
Cell Separation
Chimera
DNA - blood
DNA Primers
Female
Fetal Blood - cytology
Fetal cell microchimerism
Fetomaternal Transfusion - blood
Gestational Age
Gynecology. Andrology. Obstetrics
Humans
Linear Models
Male
Management. Prenatal diagnosis
Medical sciences
Polymerase Chain Reaction
Pregnancy
Pregnancy. Fetus. Placenta
quantitative polymerase chain reaction amplification
termination of pregnancy
Y Chromosome
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Summary:Objective: Recent reports that an association exists between fetal cell microchimerism and autoimmune disease has increased interest in the postpartum persistence of fetal cells. The purpose of this study was to determine, by means of quantitative polymerase chain reaction amplification, whether a significant fetalmaternal hemorrhage occurs after elective termination of pregnancy. Study Design: Blood samples were obtained from 23 women who underwent termination of pregnancy immediately before venipuncture; these samples were subjected to analysis by quantitative polymerase chain reaction amplification with the use of Y-chromosome primers. There were 21 male and 2 female fetuses. Results were equilibrated to 16 mL and analyzed by a weighted linear regression analysis to evaluate the correlation between detected fetal nucleated cell equivalents and gestational weeks. Results: Among the 21 known male fetuses, the median number of detected fetal nucleated cell equivalents was 1552 (range, 50-37,618). The female fetuses had no fetal nucleated cell equivalents detected. A positive dependence of male fetal nucleated cell equivalents on gestational age was shown (P
ISSN:0002-9378
1097-6868
DOI:10.1067/mob.2001.111072