Characterization of acyl-CoA-binding protein (ACBP) in the pheromone gland of the silkworm, Bombyx mori

Various fatty acyl-CoAs are involved as intermediates or precursors of sex pheromone components in the biosynthetic pathway of the pheromones in many lepidopteran insects. We have purified a 10-kDa protein from the cytosolic fraction of Bombyx mori pheromone glands by using affinity chromatography w...

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Bibliographic Details
Published in:Insect biochemistry and molecular biology 2001-04, Vol.31 (6), p.603-609
Main Authors: Matsumoto, Shogo, Yoshiga, Toyoshi, Yokoyama, Norihiro, Iwanaga, Masashi, Koshiba, Seizo, Kigawa, Takanori, Hirota, Hiroshi, Yokoyama, Shigeyuki, Okano, Kazuhiro, Mita, Kazuei, Shimada, Toru, Tatsuki, Sadahiro
Format: Article
Language:English
Subjects:
Acyl Coenzyme A
Acyl-CoA-binding protein
Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern - methods
Bombyx - chemistry
Bombyx mori
Carrier Proteins - analysis
Carrier Proteins - genetics
Cattle
Diazepam Binding Inhibitor
DNA, Complementary
Humans
Molecular Sequence Data
PBAN
Pheromone gland
Protein Structure, Secondary
Sequence Analysis, DNA
Sequence Analysis, Protein
Sequence Homology, Amino Acid
Sex Attractants
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Summary:Various fatty acyl-CoAs are involved as intermediates or precursors of sex pheromone components in the biosynthetic pathway of the pheromones in many lepidopteran insects. We have purified a 10-kDa protein from the cytosolic fraction of Bombyx mori pheromone glands by using affinity chromatography with a palmitoyl-CoA-agarose column and reversed-phase HPLC. Amino acid sequence analysis of the fragment peptides obtained from the purified protein, and a homology search, revealed that this protein was a member of acyl-CoA-binding proteins (ACBPs). MALDI-TOF mass spectral analysis of the purified protein and cloning of the gene from a pheromone gland cDNA library confirmed B. mori ACBP to be a 90 amino acid protein with 78.9% identity to that of Manduca sexta ACBP. The secondary structure of the recombinant B. mori ACBP was determined by NMR spectroscopy. Northern blot analysis demonstrated that B. mori ACBP was predominantly expressed in the pheromone gland and the corresponding transcript was expressed from the day before adult eclosion. Present results suggest that ACBP plays a significant role in the production of sex pheromones regulated by the neurohormone, pheromone biosynthesis activating neuropeptide (PBAN).
ISSN:0965-1748
1879-0240
DOI:10.1016/S0965-1748(00)00165-X