Incipient Analysis of Mesenchymal Stem-cell-derived Osteogenesis

Tissue regeneration strategies invoke cell-based therapies for effective tissue formation. Current assessment of mesenchymal stem cell (MSC) directed bone regeneration during in vivo assays is dependent on histologic determination of bone formation. It was the aim of this study to determine the rela...

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Bibliographic Details
Published in:Journal of dental research 2001-01, Vol.80 (1), p.314-320
Main Authors: Cooper, L.F., Harris, C.T., Bruder, S.P., Kowalski, R., Kadiyala, S.
Format: Article
Language:English
Subjects:
Animals
Bone Marrow Cells - cytology
Bone Marrow Cells - metabolism
Bone Marrow Transplantation
Bone Regeneration - genetics
Bone Regeneration - physiology
Colony-Forming Units Assay
Dentistry
Gene Expression Regulation, Developmental
Humans
Integrin-Binding Sialoprotein
Mesoderm - cytology
Mesoderm - metabolism
Mesoderm - transplantation
Mice
Mice, SCID
Molecular Sequence Data
Osteoblasts - cytology
Osteocalcin - biosynthesis
Osteocalcin - genetics
Osteogenesis - genetics
Osteogenesis - physiology
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
Sequence Analysis, DNA
Sialoglycoproteins - biosynthesis
Sialoglycoproteins - genetics
Stem Cell Transplantation
Stem Cells - cytology
Stem Cells - metabolism
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Summary:Tissue regeneration strategies invoke cell-based therapies for effective tissue formation. Current assessment of mesenchymal stem cell (MSC) directed bone regeneration during in vivo assays is dependent on histologic determination of bone formation. It was the aim of this study to determine the relationship between bone sialoprotein (BSP) expression and osteocalcin expression with subsequent osteogenesis occurring in MSC-based implants. RT-PCR assessment of human actin, collagen type I, BSP, and osteocalcin indicated that undifferentiated cells did not express BSP or osteocalcin. Three weeks following implantation, human BSP could be identified in RNAs isolated from the retrieved implants. For every implant from which human BSP cDNA was amplified, parallel implants harvested at 6 weeks demonstrated bone formation at the histologic level. This study confirms that, in the context of the severe combined immunodeficiency disease (SCID) mouse model, culture-expanded, cryopreserved human MSCs have osteogenic potential and demonstrates that implanted cell gene expression can reveal the early onset of bone formation.
ISSN:0022-0345
1544-0591
DOI:10.1177/00220345010800010401