Genetic differences between Escherichia coli O26 strains isolated in Brazil and in other countries

• Published in: FEMS microbiology letters 2001-03, Vol.196 (2), p.239-244
• Main Authors: Peixoto, Jean C.C., Bando, Silvia Y., Ordoñez, Juana A.G., Botelho, Beatriz A., Trabulsi, Luiz R., Moreira-Filho, Carlos A.
• Format: Article
• Language: English
• Subjects: Adhesins, Bacterial; Bacteria; Bacterial Outer Membrane Proteins - genetics; Bacterial Typing Techniques; Bacteriology; Biological and medical sciences; Brazil; Carrier Proteins; Cluster analysis; Clustering; Deoxyribonucleic acid; DNA; E coli; Enteropathogenic Escherichia coli; Epidemiology; Escherichia coli; Escherichia coli - classification; Escherichia coli - genetics; Escherichia coli - isolation & purification; Escherichia coli - pathogenicity; Escherichia coli Infections - microbiology; Escherichia coli O26; Escherichia coli Proteins; Flagellin - genetics; fliC gene; Fundamental and applied biological sciences. Psychology; Genetic Variation; Geographical distribution; Humans; Microbiology; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Phylogeny; Polymerase chain reaction; Polymerase Chain Reaction - methods; Polymorphism, Restriction Fragment Length; Random amplified polymorphic DNA; Random Amplified Polymorphic DNA Technique - methods; Restriction fragment length polymorphism polymerase chain reaction; Serotypes; Serotyping; Shiga toxin; Shiga Toxin 1 - genetics; Shiga Toxin 2 - genetics; Shiga Toxins; Shiga toxin‐producing Escherichia coli; Toxins
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1111/j.1574-6968.2001.tb10571.x

Abstract Genomic diversity among 34 strains of Escherichia coli belonging to different serotypes of the O26 serogroup – encompassing strains from different geographical origins and Shiga toxin-negative Brazilian strains – was evaluated through random amplified polymorphic DNA (RAPD) analysis. Our results indicate that Brazilian and non-Brazilian O26 strains fall under distinct but closely related differentiation clusters. RFLP-PCR analysis of the fliC gene sequence was done in order to identify the H− serotypes and served to confirm the clustering pattern obtained in the dendrogram generated from RAPD data. The epidemiological significance of these data is discussed.