Shape and quaternary structure of alpha-globulin from sesame (Sesamum indicum L.) seed as revealed by small angle x-ray scattering and quasi-elastic light scattering

The alpha-globulin from sesame seed has a molar mass of 2.7 X 10(5) g mol-1, determined by x-ray scattering, and (2.8 +/- 0.3) 10(5) g mol-1, determined by quasi-elastic light scattering. The radius of gyration RG amounts to (4.1 +/- 0.1) nm and (3.9 +/- 0.2) nm as determined by Guinier approximatio...

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Bibliographic Details
Published in:The Journal of biological chemistry 1986-09, Vol.261 (27), p.12686-12691
Main Authors: Plietz, P, Damaschun, G, Zirwer, D, Gast, K, Schwenke, K D, Prakash, V
Format: Article
Language:English
Subjects:
Alpha-Globulins
Biological and medical sciences
Brassica
Fundamental and applied biological sciences. Psychology
GLOBULINAS
GLOBULINE
GLOBULINS
Helianthus
Light
Mathematics
Molecular biophysics
Physico-chemical properties of biomolecules
PLANT PROTEINS
PROTEINAS VEGETALES
PROTEINE VEGETALE
Scattering, Radiation
Seeds - analysis
SESAMUM INDICUM
X-Rays
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Summary:The alpha-globulin from sesame seed has a molar mass of 2.7 X 10(5) g mol-1, determined by x-ray scattering, and (2.8 +/- 0.3) 10(5) g mol-1, determined by quasi-elastic light scattering. The radius of gyration RG amounts to (4.1 +/- 0.1) nm and (3.9 +/- 0.2) nm as determined by Guinier approximation and from the distribution function D(x), respectively. The molecule has a Stokes radius Rs of (5.4 +/- 0.15) nm and a maximum dimension L of (11 less than L less than 15) nm. The translational diffusion coefficient D0(20),w and the ratio of fractional coefficients f/fmin amount to (3.95 +/- 0.12) X 10(-7) cm2 s-1 and 1.25, respectively. The quaternary structure of the protein molecule is approximated by a model consisting of six spherical subunits situated at the vertices of an octahedron having the symmetry 32.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)67146-7