Biodistribution of ultrasmall iron oxide particles in the rat liver

Ferumoxtran, an ultrasmall superparamagnetic iron oxide particle, can be located in several tissue compartments in the liver, namely the extracellular space (blood and interstitium), reticuloendothelial cells, and possibly hepatocytes. To better understand the compartmental distribution of ferumoxtr...

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Bibliographic Details
Published in:Journal of magnetic resonance imaging 2001-04, Vol.13 (4), p.594-599
Main Authors: Van Beers, Bernard E., Sempoux, Christine, Materne, Roland, Delos, Monique, Smith, Anne M.
Format: Article
Language:English
Subjects:
Animals
contrast agents
contrast media
Contrast Media - pharmacokinetics
contrast media, experimental studies
Dextrans
experimental studies
Ferrosoferric Oxide
iron
Iron - pharmacokinetics
light and electron microscopy
liver
Liver - metabolism
liver, MRI
magnetic resonance (MR)
magnetic resonance (MR), contrast agents
Magnetic Resonance Imaging
Magnetite Nanoparticles
Male
Microscopy, Electron, Scanning Transmission
MRI
Oxides - pharmacokinetics
Particle Size
Rats
Rats, Wistar
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Summary:Ferumoxtran, an ultrasmall superparamagnetic iron oxide particle, can be located in several tissue compartments in the liver, namely the extracellular space (blood and interstitium), reticuloendothelial cells, and possibly hepatocytes. To better understand the compartmental distribution of ferumoxtran in the liver, we performed a longitudinal study in the rat using microscopy and magnetic resonance imaging. At light microscopy, no substantial cellular uptake of ferumoxtran was observed before one hour after injection. With a dose of 15 μmol Fe/kg, the number of ferumoxtran particles in the reticuloendothelial cells peaked between one and four hours and with a 150 μmol Fe/kg dose, it peaked between eight and 24 hours. Within hepatocytes, only sparse particles were observed with electron microscopy, at a dose of 150 μmol Fe/kg. Imaging performed up until one hour after ferumoxtran injection showed a significant increase in liver signal intensity on T1‐weighted images. These results suggest that ferumoxtran mainly acts as an extracellular agent for at least one hour in the rat and that reticuloendothelial accumulation peaks at later time points. Substantial uptake within hepatocytes did not occur. J. Magn. Reson. Imaging 2001;13:594–599. © 2001 Wiley‐Liss, Inc.
ISSN:1053-1807
1522-2586
DOI:10.1002/jmri.1083