The Mouse Putative Pheromone Receptor Was Specifically Activated by Stimulation with Male Mouse Urine

To detect the biological activity of mammalian putative pheromone receptors (VIRs and V2Rs), the mouse V1R gene was introduced into a primary culture of vomeronasal cells using the adenovirus expression system, and the response of these cells to mouse urine was analyzed by calcium imaging. These cel...

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Bibliographic Details
Published in:Journal of biochemistry (Tokyo) 2001-04, Vol.129 (4), p.509-512
Main Authors: Hagino-Yamagishi, Kimiko, Matsuoka, Masato, Ichikawa, Masumi, Wakabayashi, Yoshihiro, Mori, Yuji, Yazaki, Kazumori
Format: Article
Language:English
Subjects:
adenovirus expression system
Amino Acid Sequence
Animals
Calcium - metabolism
Calcium Signaling - drug effects
Cells, Cultured
Chemoreceptor Cells - chemistry
Chemoreceptor Cells - metabolism
Cloning, Molecular
Female
G proteins
Heterotrimeric GTP-Binding Proteins - antagonists & inhibitors
Heterotrimeric GTP-Binding Proteins - metabolism
Immunohistochemistry
Male
mammalian pheromone receptor
Mice
Molecular Sequence Data
Pertussis Toxin
Rats
Sequence Alignment
urine
Urine - physiology
Virulence Factors, Bordetella - pharmacology
vomeronasal organ
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Summary:To detect the biological activity of mammalian putative pheromone receptors (VIRs and V2Rs), the mouse V1R gene was introduced into a primary culture of vomeronasal cells using the adenovirus expression system, and the response of these cells to mouse urine was analyzed by calcium imaging. These cells specifically responded to male but not female mouse urine. This response was attenuated by pertussis toxin, a specific inhibitor of G-protein Giα/Giα coupling from receptors. Our findings indicate that a putative pheromone receptor was specifically activated by mouse urine, a major source of mouse pheromones, and suggest that Gi/Go are functionally coupled with the receptor.
ISSN:0021-924X
DOI:10.1093/oxfordjournals.jbchem.a002884