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Molecular analysis of Campoletis sonorensis virus DNA in the lepidopteran host Heliothis virescens
Department of Entomology, Texas A & M University and Texas Agricultural Experimental Station, College Station, Texas 77843-2475, U.S.A. Nucleic acid hybridization techniques were used to analyse the fate of Campoletis sonorensis virus (CsV) DNAs in naturally parasitized and virus-injected Heliot...
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Published in: | Journal of general virology 1986-09, Vol.67 (9), p.1961-1969 |
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Main Authors: | , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that cite this one |
Online Access: | Get full text |
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Summary: | Department of Entomology, Texas A & M University and Texas Agricultural Experimental Station, College Station, Texas 77843-2475, U.S.A.
Nucleic acid hybridization techniques were used to analyse the fate of Campoletis sonorensis virus (CsV) DNAs in naturally parasitized and virus-injected Heliothis virescens larvae. Viral DNA persisted in injected H. virescens larvae from 0 to 10 days post-injection but no increase in the amount of viral DNA could be detected. Similarly, no increase in the amount of viral DNA was detected in naturally parasitized H. virescens larvae before the development of the C. sonorensis pupae. However, a dramatic increase of viral DNA was detected in pharate and newly emerged (0 to 48 h) adult C. sonorensis wasps. The results from these in vivo molecular analyses suggest that CsV replication does not occur in naturally parasitized or virus-injected H. virescens and that viral replication may be restricted to tissues of C. sonorensis wasps.
Keywords: Campoletis sonorensis virus, Heliothis virescens , polydnavirus
Received 24 February 1986;
accepted 21 May 1986. |
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ISSN: | 0022-1317 1465-2099 |
DOI: | 10.1099/0022-1317-67-9-1961 |