A rapid method for the separation and analysis of carrageenan oligosaccharides released by iota- and kappa-carrageenase

Based on the improved performances in speed of chromatographic separation on Superdex-type materials (Pharmacia) compared to conventional media such as Sephadex and Bio Gel-type, a rapid size-exclusion chromatography (SEC) method was developed for the separation and analysis of carrageenan oligosacc...

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Bibliographic Details
Published in:Carbohydrate research 2001-03, Vol.331 (1), p.101-106
Main Authors: Knutsen, Svein H., Sletmoen, Marit, Kristensen, Trond, Barbeyron, Tristan, Kloareg, Bernard, Potin, Philippe
Format: Article
Language:English
Subjects:
Bacterial Proteins
Carrageenan
Carrageenan - chemistry
Carrageenan - metabolism
Chromatography, Gel - methods
Glycoside Hydrolases - metabolism
Iota-carrageenase
Isoenzymes - metabolism
Kappa-carrageenase
NMR
Nuclear Magnetic Resonance, Biomolecular
Oligosaccharides - chemistry
Oligosaccharides - isolation & purification
Pseudomonas - enzymology
Size-exclusion chromatography
Spectrometry, Mass, Electrospray Ionization
Sulphated neocarrabiose oligosaccharides
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Summary:Based on the improved performances in speed of chromatographic separation on Superdex-type materials (Pharmacia) compared to conventional media such as Sephadex and Bio Gel-type, a rapid size-exclusion chromatography (SEC) method was developed for the separation and analysis of carrageenan oligosaccharides. It was used to evaluate the elution profiles of hydrolysates produced by carrageenases specific for kappa- and iota-carrageenans. Oligosaccharide peaks ranging from di- to dodeca-saccharides were obtained in about 20 min on an analytical scale, whereas preparative runs were completed in a few hours. The method may also be used to monitor polysaccharide degradation.
ISSN:0008-6215
1873-426X
DOI:10.1016/S0008-6215(00)00324-4