MR microscopy of cobalt-labeled nerve cells and pathways in an invertebrate brain (Sepia officinalis, Cephalopoda)

This article describes a novel application of contrast‐enhanced MR microscopy to trace nerve cells and pathways through small invertebrate brains. Using the cuttlefish Sepia officinalis (Cephalopoda) as a model, the cells and pathways of one of the brain nerves were labeled with paramagnetic cobalt(...

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Bibliographic Details
Published in:Magnetic resonance in medicine 2001-04, Vol.45 (4), p.575-579
Main Authors: Quast, Michael J., Neumeister, Heike, Ezell, Edward L., Budelmann, Bernd U.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Brain - cytology
brain anatomy
Cobalt
cuttlefish
Investigative techniques, diagnostic techniques (general aspects)
magnetic resonance imaging (MRI)
Magnetic Resonance Imaging - methods
Medical sciences
Microscopy - methods
Models, Animal
Mollusca
Nervous system
Neural Pathways - cytology
neuronal tracing
Neurons - cytology
Radiodiagnosis. Nmr imagery. Nmr spectrometry
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Summary:This article describes a novel application of contrast‐enhanced MR microscopy to trace nerve cells and pathways through small invertebrate brains. Using the cuttlefish Sepia officinalis (Cephalopoda) as a model, the cells and pathways of one of the brain nerves were labeled with paramagnetic cobalt(II) ions by conventional centripetal cobalt iontophoresis. In MR microscopy, the cobalt‐labeled cell bodies and pathways became hypointense in 9.4 T spin echo images. Their course and distribution were identical with those seen with conventional histological techniques after cobalt sulphide precipitation (with or without subsequent silver intensification). Magn Reson Med 45:575–579, 2001. © 2001 Wiley‐Liss, Inc.
ISSN:0740-3194
1522-2594
DOI:10.1002/mrm.1078