Intracellular pathways involved in TNF-alpha and superoxide anion release by Abeta(1-42)-stimulated primary human macrophages

In this study, the intracellular signal transduction pathways leading to the production of TNF-alpha and superoxide anions by amyloid-beta-stimulated primary human monocyte-derived macrophages was investigated. Using Western blotting and specific inhibitors it is shown that both ERK 1/2 and p38 MAPK...

Full description

Saved in:
Bibliographic Details
Published in:Journal of neuroimmunology 2001-04, Vol.115 (1-2), p.144-151
Main Authors: Smits, H A, de Vos, N M, Wat, J W, van der Bruggen, T, Verhoef, J, Nottet, H S
Format: Article
Language:English
Subjects:
Amyloid beta-Peptides - pharmacology
Cells, Cultured
Enzyme Inhibitors - pharmacology
Humans
Macrophages - cytology
Macrophages - drug effects
Macrophages - metabolism
Mitogen-Activated Protein Kinase 1 - antagonists & inhibitors
Mitogen-Activated Protein Kinase 1 - metabolism
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinases - antagonists & inhibitors
Mitogen-Activated Protein Kinases - metabolism
p38 Mitogen-Activated Protein Kinases
Peptide Fragments - pharmacology
Protein Kinase C - antagonists & inhibitors
Protein Kinase C - metabolism
Second Messenger Systems - drug effects
Second Messenger Systems - physiology
Signal Transduction - drug effects
Signal Transduction - physiology
Superoxides - metabolism
Tumor Necrosis Factor-alpha - metabolism
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this study, the intracellular signal transduction pathways leading to the production of TNF-alpha and superoxide anions by amyloid-beta-stimulated primary human monocyte-derived macrophages was investigated. Using Western blotting and specific inhibitors it is shown that both ERK 1/2 and p38 MAPK signal transduction pathways as well as PKC are involved in the amyloid-beta-stimulated superoxide anion production. In contrast, only ERK 1/2 MAPK seems to be involved in TNF-alpha production: questioning the connection between PKC and ERK 1/2 activation. Our results suggest the use of ERK 1/2 MAPK inhibitors in the prevention of macrophage activation in the context of Alzheimer's disease.
ISSN:0165-5728