Calcium action potentials in cultured adrenocortical cells

Membrane potential has been recorded in Y-1 adrenocortical cells with intracellular microelectrodes. Resting potential averaged -68 +/- 13 mV (n = 63). Cells were silent at rest but depolarization by current pulses evoked repetitive action potentials of 80-120 mV amplitude and variable duration (bet...

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Bibliographic Details
Published in:Pflügers Archiv 1986-08, Vol.407 (2), p.163-165
Main Authors: TABARES, L, LOPEZ-BARNEO, J
Format: Article
Language:English
Subjects:
Action Potentials
Adrenal Cortex - physiology
Adrenals. Interrenals
Adrenocortical hormones. Regulation
Animals
Applied sciences
Biological and medical sciences
Calcium - physiology
Cells, Cultured
Evoked Potentials
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Membrane Potentials
Mice
Other techniques and industries
Vertebrates: endocrinology
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Summary:Membrane potential has been recorded in Y-1 adrenocortical cells with intracellular microelectrodes. Resting potential averaged -68 +/- 13 mV (n = 63). Cells were silent at rest but depolarization by current pulses evoked repetitive action potentials of 80-120 mV amplitude and variable duration (between 10-300 ms). Action potentials were unaffected by removal of external Na or addition of TTX, however they were completely abolished by substitution of external Ca by Co. In Ca-free solutions with 2.4 mM Ba, action potentials had a lower threshold and lasted for the whole duration of the current pulse.
ISSN:0031-6768
1432-2013
DOI:10.1007/BF00580669