Intra-epithelial vaccination with COPV L1 DNA by particle-mediated DNA delivery protects against mucosal challenge with infectious COPV in beagle dogs

Protection against viral challenge with canine oral papillomavirus (COPV) was achieved by immunisation via particle-mediated DNA delivery (PMDD) of a plasmid encoding the COPV L1 gene to cutaneous and oral mucosal sites in beagle dogs. The initial dose of approximately 9 μg of DNA was followed by tw...

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Bibliographic Details
Published in:Vaccine 2001-04, Vol.19 (20), p.2783-2792
Main Authors: Stanley, M.A., Moore, R.A., Nicholls, P.K., Santos, E.B., Thomsen, L., Parry, N., Walcott, S., Gough, G.
Format: Article
Language:English
Subjects:
Animals
Antibodies, Viral - blood
beta-Galactosidase - genetics
Biological and medical sciences
canine papillomavirus
Capsid - genetics
Capsid - immunology
COPV
DNA
Dogs
Fundamental and applied biological sciences. Psychology
Lymphocyte Activation
Microbiology
Papillomaviridae - genetics
Papillomaviridae - immunology
Papillomavirus Infections - prevention & control
Tumor Virus Infections - prevention & control
Vaccination
vaccine
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, DNA - immunology
Viral Vaccines - immunology
Virology
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Summary:Protection against viral challenge with canine oral papillomavirus (COPV) was achieved by immunisation via particle-mediated DNA delivery (PMDD) of a plasmid encoding the COPV L1 gene to cutaneous and oral mucosal sites in beagle dogs. The initial dose of approximately 9 μg of DNA was followed by two booster doses at 6 week intervals. A similar approach was used to vaccinate a control group of animals with plasmid DNA encoding the Hepatitis B virus S gene. Following challenge at the oral mucosa with COPV all animals vaccinated with the COPV L1 gene were protected against disease. However five of six animals in the control group developed COPV induced papillomas at the oral mucosa. Both cell-mediated lymphoproliferative and humoral antibody responses to the DNA vaccine were observed. Our data indicate that PMDD of plasmid DNA can protect against mucosal challenge with papillomavirus.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(00)00533-8