A capillary electrophoresis chip for the analysis of print and film photographic developing agents in commercial processing solutions using indirect fluorescence detection

The separation and detection of both print and film developing agents (CD‐3 and CD‐4) in photographic processing solutions using chip‐based capillary electrophoresis is presented. For simultaneous detection of both analytes under identical experimental conditions a buffer pH of 11.9 is used to parti...

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Bibliographic Details
Published in:Electrophoresis 2001-01, Vol.22 (2), p.348-354
Main Authors: Sirichai, Somsak, de Mello, Andrew J.
Format: Article
Language:English
Subjects:
Buffers
Capillary electrophoresis
Electrophoresis, Capillary - instrumentation
Equipment Design
Fluorometry - methods
Indirect fluorescence
Microchemistry - instrumentation
Microchip
Miniaturization
Photographic developer
Photography - instrumentation
Sensitivity and Specificity
Solutions
Sulfonamides - analysis
Toluene - analogs & derivatives
Toluene - analysis
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Summary:The separation and detection of both print and film developing agents (CD‐3 and CD‐4) in photographic processing solutions using chip‐based capillary electrophoresis is presented. For simultaneous detection of both analytes under identical experimental conditions a buffer pH of 11.9 is used to partially ionise the analytes. Detection is made possible by indirect fluorescence, where the ions of the analytes displace the anionic fluorescing buffer ion to create negative peaks. Under optimal conditions, both analyt‐es can be analyzed within 30 s. The limits of detection for CD‐3 and CD‐4 are 0.17 mM and 0.39 mM, respectively. The applicability of the method for the analysis of seasoned photographic processing developer solutions is also examined.
ISSN:0173-0835
1522-2683
DOI:10.1002/1522-2683(200101)22:2<348::AID-ELPS348>3.0.CO;2-4