Anti-tumor activities of chondroitinase AC and chondroitinase B: inhibition of angiogenesis, proliferation and invasion

In the current study, two specific glycosaminoglycan lyases, chondroitinase AC and chondroitinase B, were utilized to examine the roles of chondroitin sulfates and dermatan sulfate in tumor metastasis and angiogenesis. Melanoma cells (SK-MEL) or endothelial cells were treated with either medium or c...

Full description

Saved in:
Bibliographic Details
Published in:European journal of pharmacology 2001-03, Vol.416 (3), p.213-221
Main Authors: Denholm, Elizabeth M., Lin, Yong-Qing, Silver, Paul J.
Format: Article
Language:English
Subjects:
Animal tumors. Experimental tumors
Animals
Antineoplastic agents
Apoptosis
Apoptosis - drug effects
Biological and medical sciences
Cattle
Cell Division - drug effects
Cell Movement - drug effects
Cells, Cultured
Chemotherapy
Chondroitin sulfate
Chondroitin Sulfates - physiology
Chondroitinase
Chondroitinases and Chondroitin Lyases - pharmacology
Dermatan
Dermatan Sulfate - physiology
Dose-Response Relationship, Drug
Endothelium, Vascular - cytology
Endothelium, Vascular - drug effects
Experimental cardiovascular tumors
Gelatinases - metabolism
Humans
Medical sciences
Melanoma - pathology
Metastasis
Neoplasm Metastasis
Neovascularization, Pathologic
Neovascularization, Physiologic - drug effects
Pharmacology. Drug treatments
Proliferation
Tumor Cells, Cultured
Tumors
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In the current study, two specific glycosaminoglycan lyases, chondroitinase AC and chondroitinase B, were utilized to examine the roles of chondroitin sulfates and dermatan sulfate in tumor metastasis and angiogenesis. Melanoma cells (SK-MEL) or endothelial cells were treated with either medium or chondroitinase enzyme. Chondroitinase AC inhibited melanoma invasion and proliferation as well as endothelial proliferation and angiogenesis. Apoptosis of melanoma and endothelial cells, as measured by the activity of caspase-3, was also increased by chondroitinase AC, but not by chondroitinase B. Chondroitinase B inhibited endothelial and melanoma proliferation and invasion, but to a lesser extent than chondroitinase AC. Neither chondroitinase had a detectable effect on gelatinase secretion by melanoma cells. These results indicate that both chondroitin and dermatan sulfates regulate many cellular activities related to metastasis.
ISSN:0014-2999
1879-0712
DOI:10.1016/S0014-2999(01)00884-6