Zinc iodide-osmium tetroxide (ZIO) reactive sites in the extrafloral nectary of Citharexylum mirianthum Cham. (Verbenaceae)

This paper reports on a study of the zinc iodide-osmium tetroxide method (ZIO) applicability to formaldehyde-glutaraldehyde prefixed extrafloral nectary tissues of Citharexylum mirianthum Cham. (Verbenaceae). The ZIO solution impregnates the dictyosome stacks and adjacent vesicles, smooth endoplasmi...

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Bibliographic Details
Published in:Tissue & cell 2001-02, Vol.33 (1), p.72-77
Main Authors: Machado, S.R., Gregório, E.A.
Format: Article
Language:English
Subjects:
Binding Sites
Magnoliopsida - anatomy & histology
Magnoliopsida - ultrastructure
Organelles - ultrastructure
Osmium Tetroxide - pharmacology
Plant Epidermis - ultrastructure
Plant Leaves - ultrastructure
Zinc Compounds - pharmacology
ZIO method, ultrastructure, extrafloral nectary, Citharexylum mirianthum, Verbenaceae
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Summary:This paper reports on a study of the zinc iodide-osmium tetroxide method (ZIO) applicability to formaldehyde-glutaraldehyde prefixed extrafloral nectary tissues of Citharexylum mirianthum Cham. (Verbenaceae). The ZIO solution impregnates the dictyosome stacks and adjacent vesicles, smooth endoplasmic reticulum, nuclear envelope, multivesicular bodies, and peroxisomes. The use of this method greatly facilitates the observation and recognition of organelles in each nectary region. It also allows the correlation between structure and function in nectariferous cells.
ISSN:0040-8166
1532-3072
DOI:10.1054/tice.2000.0157