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Molecular cloning and functional characterization of two kinds of betaine-aldehyde dehydrogenase in betaine-accumulating mangrove Avicennia marina (Forsk.) Vierh

Glycinebetaine is an important osmoprotectant in bacteria, plants, and animals, but only little information is available on the synthesis of glycinebetaine in tree plants. Among four mangrove species, glycinebetaine could be detected only in Avicennia marina. Pinitol was the main osmoprotectant in t...

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Published in:Plant molecular biology 2001-02, Vol.45 (3), p.353-363
Main Authors: Hibino, T, Meng, Y L, Kawamitsu, Y, Uehara, N, Matsuda, N, Tanaka, Y, Ishikawa, H, Baba, S, Takabe, T, Wada, K, Ishii, T
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container_end_page 363
container_issue 3
container_start_page 353
container_title Plant molecular biology
container_volume 45
creator Hibino, T
Meng, Y L
Kawamitsu, Y
Uehara, N
Matsuda, N
Tanaka, Y
Ishikawa, H
Baba, S
Takabe, T
Wada, K
Ishii, T
description Glycinebetaine is an important osmoprotectant in bacteria, plants, and animals, but only little information is available on the synthesis of glycinebetaine in tree plants. Among four mangrove species, glycinebetaine could be detected only in Avicennia marina. Pinitol was the main osmoprotectant in the other three species. The level of glycinebetaine in A. marina increased under high salinity. Betaine-aldehyde dehydrogenase (BADH) was detected in all four species, but choline monooxygenase could not be detected. A cDNA library was constructed from the leaves of A. marina. Two kinds of BADH cDNAs were isolated, one homologous to the spinach chloroplast BADH, and the other with unique residues SKL at the end of C-terminus. The BADH transcription levels of the former were higher than those of the latter. The levels of the former BADH increased at high salinity whereas those of the latter were independent of salinity. BADHs were expressed in Escherichia coli and purified. Two kinds of A. marina BADHs exhibited similar kinetic and stability properties, but were significantly different from those of spinach BADH. A. marina BADHs efficiently catalyzed the oxidation of betainealdehyde, but not the oxidation of omega-aminoaldehydes and were more stable at high temperature than the spinach BADH.
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Vierh</title><source>Springer Nature</source><creator>Hibino, T ; Meng, Y L ; Kawamitsu, Y ; Uehara, N ; Matsuda, N ; Tanaka, Y ; Ishikawa, H ; Baba, S ; Takabe, T ; Wada, K ; Ishii, T</creator><creatorcontrib>Hibino, T ; Meng, Y L ; Kawamitsu, Y ; Uehara, N ; Matsuda, N ; Tanaka, Y ; Ishikawa, H ; Baba, S ; Takabe, T ; Wada, K ; Ishii, T</creatorcontrib><description>Glycinebetaine is an important osmoprotectant in bacteria, plants, and animals, but only little information is available on the synthesis of glycinebetaine in tree plants. Among four mangrove species, glycinebetaine could be detected only in Avicennia marina. Pinitol was the main osmoprotectant in the other three species. The level of glycinebetaine in A. marina increased under high salinity. Betaine-aldehyde dehydrogenase (BADH) was detected in all four species, but choline monooxygenase could not be detected. A cDNA library was constructed from the leaves of A. marina. Two kinds of BADH cDNAs were isolated, one homologous to the spinach chloroplast BADH, and the other with unique residues SKL at the end of C-terminus. The BADH transcription levels of the former were higher than those of the latter. The levels of the former BADH increased at high salinity whereas those of the latter were independent of salinity. BADHs were expressed in Escherichia coli and purified. Two kinds of A. marina BADHs exhibited similar kinetic and stability properties, but were significantly different from those of spinach BADH. 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Vierh</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>Glycinebetaine is an important osmoprotectant in bacteria, plants, and animals, but only little information is available on the synthesis of glycinebetaine in tree plants. Among four mangrove species, glycinebetaine could be detected only in Avicennia marina. Pinitol was the main osmoprotectant in the other three species. The level of glycinebetaine in A. marina increased under high salinity. Betaine-aldehyde dehydrogenase (BADH) was detected in all four species, but choline monooxygenase could not be detected. A cDNA library was constructed from the leaves of A. marina. Two kinds of BADH cDNAs were isolated, one homologous to the spinach chloroplast BADH, and the other with unique residues SKL at the end of C-terminus. The BADH transcription levels of the former were higher than those of the latter. 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ispartof Plant molecular biology, 2001-02, Vol.45 (3), p.353-363
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subjects Aldehyde Oxidoreductases - genetics
Aldehyde Oxidoreductases - metabolism
Amino Acid Sequence
Avicennia marina
Betaine - metabolism
Betaine-Aldehyde Dehydrogenase
Calcium Chloride - pharmacology
Carbohydrate Metabolism
Cloning
Cloning, Molecular
Dehydrogenase
DNA, Complementary - chemistry
DNA, Complementary - genetics
Dose-Response Relationship, Drug
E coli
Enzyme Stability
Escherichia coli
gamma-Aminobutyric Acid - metabolism
Gene Expression Regulation, Enzymologic - drug effects
Gene Expression Regulation, Plant - drug effects
glycinebetaine
High temperature
Hot Temperature
Isoenzymes - genetics
Molecular Sequence Data
Osmolar Concentration
Oxidation
Oxidation-Reduction - drug effects
Oxygenases - metabolism
Plant Leaves - drug effects
Plant Leaves - enzymology
Plant Leaves - genetics
Plants, Medicinal - enzymology
Plants, Medicinal - genetics
Plants, Medicinal - metabolism
Potassium Chloride - pharmacology
Proline - metabolism
RNA, Messenger - drug effects
RNA, Messenger - genetics
RNA, Messenger - metabolism
Salinity
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Sodium Chloride - pharmacology
Species Specificity
Spinacia oleracea - enzymology
Substrate Specificity
Tissue Distribution
title Molecular cloning and functional characterization of two kinds of betaine-aldehyde dehydrogenase in betaine-accumulating mangrove Avicennia marina (Forsk.) Vierh
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