Cloning and characterization of Rhesus monkey neuropeptide Y receptor subtypes

Neuropeptide Y (NPY) is a 36 amino acid peptide that is abundant in the brain and peripheral nervous system. NPY has a variety of effects when administered into the brain including a pronounced feeding effect, anxiolysis, regulation of neuroendocrine axes and inhibition of neurotransmitter release....

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Bibliographic Details
Published in:Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2001-03, Vol.22 (3), p.343-350
Main Authors: Gehlert, Donald R., Yang, Peiyi, George, Carolyn, Wang, Yi, Schober, Douglas, Gackenheimer, Susan, Johnson, Dwayne, Beavers, Lisa S., Gadski, Robert A., Baez, Melvyn
Format: Article
Language:English
Subjects:
7TM
Amino Acid Sequence
Animals
Binding, Competitive
Cloning, Molecular
Conserved Sequence
DNA, Complementary - metabolism
Dose-Response Relationship, Drug
G-protein coupled
Humans
Kinetics
Macaca mulatta
Molecular Sequence Data
Peptide YY
Polymerase Chain Reaction
Protein Structure, Tertiary
Radioligand Assay
Receptors, Neuropeptide Y - chemistry
Receptors, Neuropeptide Y - genetics
Y2 receptor
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Summary:Neuropeptide Y (NPY) is a 36 amino acid peptide that is abundant in the brain and peripheral nervous system. NPY has a variety of effects when administered into the brain including a pronounced feeding effect, anxiolysis, regulation of neuroendocrine axes and inhibition of neurotransmitter release. These effects are mediated by up to 6 G protein coupled receptors designated Y1, Y2, Y3, Y4, Y5 and y6. To better understand the phylogeny and pharmacology of NPY in non-human primates, we have cloned and expressed the NPY Y1, Y2 and Y5 receptor subtypes from the Rhesus monkey. No cDNA sequence encoding a Y4 receptor was found suggesting substantial sequence differences when compared to the human sequence. Comparison of these sequences with those from human indicated strong sequence conservation of Y1, Y2 and Y5 between the two species. The displacement of 125I-PYY binding to the Rhesus monkey and human receptors by various peptides was compared to evaluate the pharmacology of the two species. Similar pharmacologies were noted across the species at the various receptor subtypes. These results indicate the Rhesus monkey and human NPY receptor subtypes have a close amino acid sequence conservation and that the peptide recognition domains are conserved as well.
ISSN:0196-9781
1873-5169
DOI:10.1016/S0196-9781(01)00336-9