Detection of Salmonella enteritidis in shell and liquid eggs using enrichment and plating

Detection methods using various enrichment and plating media and immunoconcentration for Salmonella enteritidis in shell and liquid eggs were evaluated. For liquid egg samples naturally contaminated with S. enteritidis, pre-enrichment in 225 ml of buffered peptone water with cysteine followed by sel...

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Bibliographic Details
Published in:International journal of food microbiology 2001-03, Vol.64 (3), p.395-399
Main Authors: Hara-Kudo, Yukiko, Kumagai, Susumu, Masuda, Takashi, Goto, Koukichi, Ohtsuka, Kayoko, Masaki, Hiroyuki, Tanaka, Hiroyuki, Tanno, Kenji, Miyahara, Michiko, Konuma, Hirotaka
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Colony Count, Microbial
Culture Media
Detection
Egg
Egg and egg product industries
Egg Shell - microbiology
Eggs - microbiology
Enrichment
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
Immunomagnetic Separation
Plating
Salmonella enteritidis
Salmonella enteritidis - isolation & purification
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Summary:Detection methods using various enrichment and plating media and immunoconcentration for Salmonella enteritidis in shell and liquid eggs were evaluated. For liquid egg samples naturally contaminated with S. enteritidis, pre-enrichment in 225 ml of buffered peptone water with cysteine followed by selective enrichment in 10 ml of tetrathionate broth was the superior, resulting in the detection of S. enteritidis in all samples on six of the seven types of selective agar substrate investigated. This enrichment procedure also enabled detection of S. enteritidis in most of artificially inoculated shell egg and pasteurized liquid egg samples.
ISSN:0168-1605
1879-3460
DOI:10.1016/S0168-1605(00)00475-X