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Detection of Salmonella enteritidis in shell and liquid eggs using enrichment and plating
Detection methods using various enrichment and plating media and immunoconcentration for Salmonella enteritidis in shell and liquid eggs were evaluated. For liquid egg samples naturally contaminated with S. enteritidis, pre-enrichment in 225 ml of buffered peptone water with cysteine followed by sel...
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Published in: | International journal of food microbiology 2001-03, Vol.64 (3), p.395-399 |
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container_end_page | 399 |
container_issue | 3 |
container_start_page | 395 |
container_title | International journal of food microbiology |
container_volume | 64 |
creator | Hara-Kudo, Yukiko Kumagai, Susumu Masuda, Takashi Goto, Koukichi Ohtsuka, Kayoko Masaki, Hiroyuki Tanaka, Hiroyuki Tanno, Kenji Miyahara, Michiko Konuma, Hirotaka |
description | Detection methods using various enrichment and plating media and immunoconcentration for
Salmonella enteritidis in shell and liquid eggs were evaluated. For liquid egg samples naturally contaminated with
S. enteritidis, pre-enrichment in 225 ml of buffered peptone water with cysteine followed by selective enrichment in 10 ml of tetrathionate broth was the superior, resulting in the detection of
S. enteritidis in all samples on six of the seven types of selective agar substrate investigated. This enrichment procedure also enabled detection of
S. enteritidis in most of artificially inoculated shell egg and pasteurized liquid egg samples. |
doi_str_mv | 10.1016/S0168-1605(00)00475-X |
format | article |
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Salmonella enteritidis in shell and liquid eggs were evaluated. For liquid egg samples naturally contaminated with
S. enteritidis, pre-enrichment in 225 ml of buffered peptone water with cysteine followed by selective enrichment in 10 ml of tetrathionate broth was the superior, resulting in the detection of
S. enteritidis in all samples on six of the seven types of selective agar substrate investigated. This enrichment procedure also enabled detection of
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Salmonella enteritidis in shell and liquid eggs were evaluated. For liquid egg samples naturally contaminated with
S. enteritidis, pre-enrichment in 225 ml of buffered peptone water with cysteine followed by selective enrichment in 10 ml of tetrathionate broth was the superior, resulting in the detection of
S. enteritidis in all samples on six of the seven types of selective agar substrate investigated. This enrichment procedure also enabled detection of
S. enteritidis in most of artificially inoculated shell egg and pasteurized liquid egg samples.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Colony Count, Microbial</subject><subject>Culture Media</subject><subject>Detection</subject><subject>Egg</subject><subject>Egg and egg product industries</subject><subject>Egg Shell - microbiology</subject><subject>Eggs - microbiology</subject><subject>Enrichment</subject><subject>Food industries</subject><subject>Food microbiology</subject><subject>Fundamental and applied biological sciences. 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Salmonella enteritidis in shell and liquid eggs were evaluated. For liquid egg samples naturally contaminated with
S. enteritidis, pre-enrichment in 225 ml of buffered peptone water with cysteine followed by selective enrichment in 10 ml of tetrathionate broth was the superior, resulting in the detection of
S. enteritidis in all samples on six of the seven types of selective agar substrate investigated. This enrichment procedure also enabled detection of
S. enteritidis in most of artificially inoculated shell egg and pasteurized liquid egg samples.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>11294363</pmid><doi>10.1016/S0168-1605(00)00475-X</doi><tpages>5</tpages></addata></record> |
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subjects | Animals Biological and medical sciences Colony Count, Microbial Culture Media Detection Egg Egg and egg product industries Egg Shell - microbiology Eggs - microbiology Enrichment Food industries Food microbiology Fundamental and applied biological sciences. Psychology Immunomagnetic Separation Plating Salmonella enteritidis Salmonella enteritidis - isolation & purification |
title | Detection of Salmonella enteritidis in shell and liquid eggs using enrichment and plating |
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