Secretion of cytokines by uroepithelial cells stimulated by Escherichia coli and Citrobacter spp

Urinary tract epithelial cells (T 24/83) are able to express interleukin (IL)-6, IL-8, platelet-derived growth factor (PDGF) and tumour necrosis factor-α, but not IL-1β, IL-2, IL-4 and IL-10 in response to an infection with uropathogenic bacteria. The process of cytokine secretion is time dependent,...

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Bibliographic Details
Published in:International journal of antimicrobial agents 2001-04, Vol.17 (4), p.253-258
Main Authors: Fünfstück, Reinhard, Franke, Sybille, Hellberg, Matthias, Ott, Undine, Knöfel, Brigitte, Straube, Eberhard, Sommer, Manfred, Hacker, Jörg
Format: Article
Language:English
Subjects:
Citrobacter
Citrobacter - pathogenicity
Cytokine activation
Cytokines - secretion
Enterobacteriaceae Infections - physiopathology
Enzyme-Linked Immunosorbent Assay
Escherichia coli
Escherichia coli - pathogenicity
Escherichia coli Infections - physiopathology
Humans
Interleukin-6 - secretion
Interleukin-8 - secretion
Platelet-Derived Growth Factor - secretion
Tumor Cells, Cultured
Tumor Necrosis Factor-alpha - secretion
Urinary Bladder Neoplasms
Urinary tract infection
Urinary Tract Infections - metabolism
Urinary Tract Infections - microbiology
Uroepithelial cells
Urogenital System - metabolism
Urogenital System - microbiology
Urothelium - immunology
Urothelium - microbiology
Urothelium - secretion
Virulence
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Summary:Urinary tract epithelial cells (T 24/83) are able to express interleukin (IL)-6, IL-8, platelet-derived growth factor (PDGF) and tumour necrosis factor-α, but not IL-1β, IL-2, IL-4 and IL-10 in response to an infection with uropathogenic bacteria. The process of cytokine secretion is time dependent, with a significant increase in the cytokine activity after 60 min. The expression of virulence factors of the bacteria does not seem to play a role. The interaction between bacterial products (e.g. lipopolysaccharide) and/or bacterial adhesion mediated by adhesins and specific receptor molecules of cell surfaces may be responsible for the activity of mediator protein expression in the epithelial cells. The release of PDGF and IL-8 was found to be higher when due to Escherichia coli HB 101 (rough form) than that caused by other bacterial strains. Citrobacter CB 3009 provoked the highest level of IL-6. The PDGF level correlated significantly with IL-6 and IL-8 values ( P
ISSN:0924-8579
1872-7913
DOI:10.1016/S0924-8579(01)00301-6