Molecular Epidemiology of Bordetella pertussis by Pulsed-Field Gel Electrophoresis Profile: Cincinnati, 1989–1996

Reported cases of pertussis have increased in the United States, with peaks occurring every few years. Bordetella pertussis isolates collected in Cincinnati from 1989 to 1996 were analyzed with pulsed-field gel electrophoresis (PFGE), to evaluate trends. Among 496 isolates, 30 PFGE profiles were ide...

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Bibliographic Details
Published in:The Journal of infectious diseases 2001-05, Vol.183 (9), p.1360-1367
Main Authors: Bisgard, Kristine M., Christie, Celia D. C., Reising, Shirley F., Sanden, Gary N., Cassiday, Pamela K., Gomersall, Claire, Wattigney, Wendy A., Roberts, Nancy E., Strebel, Peter M.
Format: Article
Language:English
Subjects:
Age Factors
Bacterial Typing Techniques
Bacteriology
Biological and medical sciences
Bordetella pertussis
Bordetella pertussis - classification
Bordetella pertussis - genetics
Bordetella pertussis - isolation & purification
Child
Child, Preschool
Disease Outbreaks
Disease transmission
DNA Restriction Enzymes
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
Electrophoresis
Electrophoresis, Gel, Pulsed-Field
Epidemics
Epidemiology
Female
Fundamental and applied biological sciences. Psychology
Gels
Humans
Indiana - epidemiology
Infant
Infant, Newborn
Infectious diseases
Kentucky - epidemiology
Major Articles
Male
Microbiology
Molecular Epidemiology
Ohio - epidemiology
Preventive medicine
Seasons
USA, Ohio
Vaccination
Virology
Whooping cough
Whooping Cough - epidemiology
Whooping Cough - microbiology
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Description
Summary:Reported cases of pertussis have increased in the United States, with peaks occurring every few years. Bordetella pertussis isolates collected in Cincinnati from 1989 to 1996 were analyzed with pulsed-field gel electrophoresis (PFGE), to evaluate trends. Among 496 isolates, 30 PFGE profiles were identified; 32% were CYXXI-010, the profile that predominated each year. Eighteen profiles (198 strains) were identified in 1989–1992, 20 profiles (197 strains) were identified during the 1993 epidemic, and 11 profiles (101 strains) were identified in 1994–1996. From 1989 to 1996, among 42 patients, isolates from household members in 17 (89%) of 19 households had concordant PFGE profiles. There was no association between PFGE profile and seasonality, age, and hospitalization or pneumonia in infants
ISSN:0022-1899
1537-6613
DOI:10.1086/319858