Interleukin 12 and antigen independently induce substance P receptor expression in T cells in murine schistosomiasis mansoni

• Published in: The FASEB journal 2001-04, Vol.15 (6), p.950-957
• Main Authors: Blum, A M, Metwali, A, Crawford, C, Li, J, Qadir, K, Elliott, D E, Weinstock, J V
• Format: Article
• Language: English
• Subjects: Animals; Antigens, Helminth - pharmacology; Disease Models, Animal; Granuloma - metabolism; Helminth Proteins - pharmacology; Interferon-gamma - metabolism; Interleukin-12 - pharmacology; Mice; Mice, Inbred C57BL; Mice, Inbred CBA; Receptors, Neurokinin-1 - genetics; Receptors, Neurokinin-1 - metabolism; RNA, Messenger - metabolism; Schistosomiasis mansoni - metabolism; Schistosomiasis mansoni - pathology; Spleen - metabolism; Spleen - pathology; T-Lymphocytes - drug effects; T-Lymphocytes - metabolism
• ISSN: 0892-6638; 1530-6860
• DOI: 10.1096/fj.00-0379

Substance P (SP) regulates interferon-gamma (IFN-gamma) production through interaction with the SP receptor NK1 (SPr) on T cells at sites of inflammation. Using murine schistosomiasis, we evaluated whether SPr expression was subject to immunoregulation. Splenocytes from schistosome-infected mice cultured for < or =18 h did not express SPr, as determined by quantitative polymerase chain reaction assay. However, exposure to schistosome egg antigen (SEA) for < or =4 h induced strong receptor expression. Experiments using splenocytes fractionated with antibody-coupled, paramagnetic beads showed that induction localized exclusively to T cells. Receptor protein expression was confirmed with Western blot. Interleukin 12 (IL-12) also induced strong T-cell SPr expression. Both SEA and IL-12 remained strong inducers of T-cell SPr in lymphocytes from the IL-12 (p40) and IFN-gamma R double-knockout mouse, which suggested that SEA did not require IL-12 to induce SPr and that both worked independently of IFN-gamma. Splenocytes from wild-type mice cultured with SEA and neutralizing anti-IL-12 monoclonal antibody (mAb) also showed SPr induction. However, anti-Ia mAb inhibited SEA induction of SPR: Thus, SPr is inducible on T cells. SEA induces SPr through interaction with T-cell receptor (TCR), independently of IL-12 and IFN-gamma. IL-12 induces SPr independently of TCR activation and IFN-gamma expression. SP and its receptor, which regulate IFN-gamma production, are probably part of the IL-12-Th1 circuit.