A highly conserved vascular permeability factor secreted by a variety of human and rodent tumor cell lines

We have previously reported that rodent tumor cell lines secrete a potent vascular permeability factor with a molecular weight of 34,000-42,000 (Senger et al. Tumor cells secrete a vascular permeability factor that promotes accumulation of ascites fluid. Science (Wash. DC), 219: 983-985, 1983). This...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1986-11, Vol.46 (11), p.5629-5632
Main Authors: SENGER, D. R, PERRUZZI, C. A, FEDER, J, DVORAK, H. F
Format: Article
Language:English
Subjects:
Animals
Antigen-Antibody Reactions
Applied sciences
Biological and medical sciences
Capillary Permeability
Cell Line
Culture Media
Exact sciences and technology
General aspects (metabolism, cell proliferation, established cell line...)
Humans
Lymphokines - immunology
Lymphokines - secretion
Medical sciences
Molecular Weight
Neoplasms, Experimental - secretion
Other techniques and industries
Rats
Tumor cell
Tumors
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors
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Summary:We have previously reported that rodent tumor cell lines secrete a potent vascular permeability factor with a molecular weight of 34,000-42,000 (Senger et al. Tumor cells secrete a vascular permeability factor that promotes accumulation of ascites fluid. Science (Wash. DC), 219: 983-985, 1983). This tumor-secreted vascular permeability factor (VPF) causes a rapid and completely reversible increase in microvascular permeability in the species (guinea pig or rat) from which the tumors were derived without causing mast cell degranulation or endothelial cell damage or exciting an inflammatory cell infiltrate. This VPF may be responsible, at least in part, for the increased permeability which is commonly displayed by solid and ascites tumor vessels. We have now examined 7 human tumor cell lines and have determined that 5 of them also secrete this same VPF. Antibody raised to guinea pig line 10 VPF neutralized more than 90% of the vascular permeability-increasing activity secreted by these 5 human tumor lines. Furthermore, VPFs from both guinea pig and human tumor sources bound to and were eluted similarly from immobilized heparin and comigrated identically on sodium dodecyl sulfate-polyacrylamide gels. Finally, 2 tumorigenic (in nude mice) human cell lines were found to secrete at least 14-fold more VPF than their directly matched, nontumorigenic counterparts, suggesting that elevated expression of this permeability factor may correlate with neoplastic transformation. These data suggest that a broad spectrum of tumor cells from several species, including humans, secretes a highly conserved molecule that enhances local vascular permeability and that this function may be important for tumor growth.
ISSN:0008-5472
1538-7445