Structural changes of human serum albumin immobilized on chromatographic supports: a high-performance liquid chromatography and Fourier-transform infrared spectroscopy study

Chiral stationary phases obtained by immobilization of HSA on [C8] and [C18] reversed-phases and on poly(1-vinylimidazole)-coated silica were tested to resolve dl-tryptophan, N-benzoyl- dl-phenylalanine, RS-oxazepam and RS-warfarin racemic mixtures. Parameters of enantioselectivity measured in HPLC...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 2001-03, Vol.753 (1), p.101-113
Main Authors: Millot, M.C, Servagent-Noinville, S, Taleb, N.L, Baron, M.H, Revault, M, Sébille, B
Format: Article
Language:English
Subjects:
Albumins - chemistry
Analysis
Biological and medical sciences
Chemical Sciences
Chromatography, High Pressure Liquid - methods
General pharmacology
Human serum albumin
Humans
Medical sciences
Pharmacology. Drug treatments
Protein Conformation
Spectroscopy, Fourier Transform Infrared - methods
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Summary:Chiral stationary phases obtained by immobilization of HSA on [C8] and [C18] reversed-phases and on poly(1-vinylimidazole)-coated silica were tested to resolve dl-tryptophan, N-benzoyl- dl-phenylalanine, RS-oxazepam and RS-warfarin racemic mixtures. Parameters of enantioselectivity measured in HPLC are correlated to structural and solvation states for adsorbed HSA, evaluated by FTIR spectroscopy. HSA immobilized on [PVI]-anion-exchangers is highly selective. HSA molecules are not self-associated, only unfolded for a small hydrophobic helix. The HSA-coated reversed-phases have a lower selectivity. Unfolding is larger but the indole-benzodiazepine chiral site is preserved and remains accessible.
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(00)00424-2