Altered Growth Kinetics Precede Calcium-Induced Differentiation in Mouse Epidermal Cells

Primary cultures of newborn mouse epidermal cells proliferate rapidly and with a high growth fraction for several months when grown in medium with low calcium (0.02 to 0.1 mM). Addition of calcium to levels generally used in culture medium (1.2 mM) was followed by rapid changes in the pattern of pro...

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Bibliographic Details
Published in:In Vitro Cellular & Developmental Biology 1986-06, Vol.22 (6), p.332-336
Main Authors: ELGJO, K, HENNINGS, H, CLAUSEN, O. P. F
Format: Article
Language:English
Subjects:
Animal cells
Animals
Biological and medical sciences
Biotechnology
Calcium
Calcium - pharmacology
Cell culture techniques
Cell cultures. Hybridization. Fusion
Cell cycle
Cell Differentiation - drug effects
Cell Division - drug effects
Cell growth
Cells, Cultured
Cultured cells
Diploidy
DNA - analysis
Epidermal cells
Epidermis - analysis
Epidermis - cytology
Epidermis - drug effects
Establishment of new cell lines, improvement of cultural methods, mass cultures
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Interphase
Kinetics
Methods. Procedures. Technologies
Mice
Mice, Inbred BALB C
Molecular and cellular biology
Tetraploidy
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Summary:Primary cultures of newborn mouse epidermal cells proliferate rapidly and with a high growth fraction for several months when grown in medium with low calcium (0.02 to 0.1 mM). Addition of calcium to levels generally used in culture medium (1.2 mM) was followed by rapid changes in the pattern of proliferation. By using a combination of technics (a stathmokinetic method, autoradiography, [3H]thymidine incorporation into DNA, DNA flow cytometry) it was found that cell flux was blocked for 5 to 6 h, followed by a short rise in the mitotic rate at 10 h, and a gradual fall in all growth parameters until about 32 h after the calcium switch. There was no accumulation of cells in any particular cell cycle phase. The results indicate that the calcium switch is followed by a strong reduction in cell flux from G1whereas the majority of the cells that had left G1at the time of the switch completed one cell division before cessation of all proliferative activity. Both before and after the switch the primary epidermal cultures consisted of one diploid and one tetraploid G1DNA stemline that seemed to react in the same way to calcium.
ISSN:0883-8364
2327-431X
1475-2689
DOI:10.1007/BF02623407