Kinetics of erythromycin uptake and release by human lymphocytes and polymorphonuclear leucocytes

The uptake of 14C-labelled erythromycin by human lymphocytes and polymorpho-nuclear leucocytes was studied. Erythromycin was concentrated by the cells. The amount of accumulated erythromycin was correlated with the cell count and was found to increase with alkaline pH and with increasing temperature...

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Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 1986-07, Vol.18 (1), p.73-82
Main Authors: Dette, G. A., Knothe, H.
Format: Article
Language:English
Subjects:
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Biological and medical sciences
erythromycin
Erythromycin - blood
Humans
Hydrogen-Ion Concentration
Kinetics
leukocytes (polymorphonuclear)
lymphocytes
Lymphocytes - metabolism
man
Medical sciences
Neutrophils - metabolism
Pharmacology. Drug treatments
Temperature
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Summary:The uptake of 14C-labelled erythromycin by human lymphocytes and polymorpho-nuclear leucocytes was studied. Erythromycin was concentrated by the cells. The amount of accumulated erythromycin was correlated with the cell count and was found to increase with alkaline pH and with increasing temperature of the incubation medium. The uptake of erythromycin could be reduced by compounds which inhibit cell respiration, glycolysis and (Na+–K+)membrane ATPase. Furthermore, the uptake was saturable and followed Michaelis-Menten kinetics (Km=0.7 and 1.6 mM for lymphocytes and polymorphonuclear leucocytes, respectively). The intracellular concentrations cannot be explained by the principle of non ionic diffusion. It is suggested that erythromycin is actively transported via the nucleoside transport system. The accumulated erythromycin was rapidly released when the cells were washed and re-incubated in antibiotic-free medium. Probably, no strong intracellular binding takes place.
ISSN:0305-7453
1460-2091
DOI:10.1093/jac/18.1.73