The nine-banded armadillo ( Dasypus novemcinctus) is naturally infected with Sarcocystis neurona

Sarcocysts were dissected from the tongue of a nine-banded armadillo ( Dasypus novemcinctus). DNA was extracted and characterised by PCR amplification followed by restriction fragment length polymorphism analysis and nucleotide sequencing. A total of 1879 nucleotides were compared; the sarcocyst DNA...

Full description

Saved in:
Bibliographic Details
Published in:International journal for parasitology 2001-04, Vol.31 (4), p.325-329
Main Authors: Tanhauser, S.M, Cheadle, M.A, Massey, E.T, Mayer, B.A, Schroedter, D.E, Dame, J.B, Greiner, E.C, MacKay, R.J
Format: Article
Language:English
Subjects:
Animal and plant ecology
Animal, plant and microbial ecology
Animals
Antibodies, Protozoan - blood
Armadillo
Armadillos - blood
Armadillos - parasitology
Autoecology
Biological and medical sciences
Blotting, Western - veterinary
Dasypus novemcinctus
DNA, Protozoan - isolation & purification
Equine protozoal myeloencephalitis
Female
Fundamental and applied biological sciences. Psychology
Host-Parasite Interactions - physiology
Intermediate host
Male
Mammalia
Muscle, Skeletal - parasitology
Opossum
Polymerase Chain Reaction - veterinary
Polymorphism, Restriction Fragment Length
Psychoanalysis
Psychoanalytic cure
Psychology. Psychoanalysis. Psychiatry
Sarcocystis - genetics
Sarcocystis - physiology
Sarcocystis neurona
Sarcocystosis - transmission
Sarcocystosis - veterinary
sarcocysts
Sequence Analysis, DNA
Tongue - parasitology
Vertebrata
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Sarcocysts were dissected from the tongue of a nine-banded armadillo ( Dasypus novemcinctus). DNA was extracted and characterised by PCR amplification followed by restriction fragment length polymorphism analysis and nucleotide sequencing. A total of 1879 nucleotides were compared; the sarcocyst DNA sequence was identical to that reported for Sarcocystis neurona. DNA was extracted from the sarcocysts of five more nine-banded armadillos. A 254-nucleotide sequence was determined for each and found to be identical to S. neurona. Western blot techniques for detection of anti- S. neurona antibody were developed for use with armadillo plasma and samples from 19 wild-caught and 17 captive-raised armadillos were examined. Whereas all of the 19 wild-caught armadillos had antibodies to S. neurona, only one of 17 captive-raised armadillos did. These results suggest that the nine-banded armadillo are naturally infected with S. neurona.
ISSN:0020-7519
1879-0135
DOI:10.1016/S0020-7519(01)00178-3