Expression profiling in knockout mice: lymphotoxin versus tumor necrosis factor in the maintenance of splenic microarchitecture

Expression profiling provides a powerful approach to define the underlying molecular mechanisms in disease. Several techniques referred collectively to as gene profiling may be also helpful in the analysis of the phenotype of mice with targeted mutations, especially if applied to distinct histologic...

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Bibliographic Details
Published in:Cytokine & growth factor reviews 2001-03, Vol.12 (1), p.107-119
Main Authors: Shakhov, Alexander N, Nedospasov, Sergei A
Format: Article
Language:English
Subjects:
Animals
Antigens, Surface
Chemokines - genetics
Chemokines - metabolism
Cytokines
Gene arrays
Gene Expression Profiling - methods
Group II Phospholipases A2
Lymphocytes - metabolism
Lymphotoxin-alpha - genetics
Lymphotoxin-alpha - metabolism
Membrane Glycoproteins - genetics
Membrane Glycoproteins - metabolism
Mice
Mice, Knockout
Milk Proteins
Mucins - genetics
Mucins - metabolism
Phospholipases A - genetics
Phospholipases A - metabolism
Receptors, Immunologic - genetics
Receptors, Immunologic - metabolism
Signal Transduction
Spleen
Spleen - pathology
Spleen - physiology
Spleen - ultrastructure
Subtractive cloning
Tumor Necrosis Factor-alpha - deficiency
Tumor Necrosis Factor-alpha - genetics
Tumor Necrosis Factor-alpha - metabolism
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Summary:Expression profiling provides a powerful approach to define the underlying molecular mechanisms in disease. Several techniques referred collectively to as gene profiling may be also helpful in the analysis of the phenotype of mice with targeted mutations, especially if applied to distinct histological compartments, to specific cell types or to evaluate the effect of specific challenges, such as infection. Here we review several of the existing techniques applicable to genetic knockout studies, and share our experience from the study of mice with tumor necrosis factor (TNF) and lymphotoxin (LT) deficiencies, with specific emphasis on the distinction between TNF- and LT-mediated signalling pathways in vivo. Gene expression profiling analysis of TNF/LT-deficient mice supports the notion that TNF and LT, originally discovered as distinct biological activities, manifest both distinct and redundant functions in vivo.
ISSN:1359-6101
DOI:10.1016/S1359-6101(01)00004-1