Effect of food manipulation on the neuropeptide Y neuronal system in the diencephalon of ewes

The expression of neuropeptide Y (NPY), which is involved in the neuromodulatory function associated with animal nutrition, growth and reproduction, depends on the nutritional status. However, the roles of individual components of food are not fully recognised. In this study, we evaluated the effect...

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Bibliographic Details
Published in:Journal of chemical neuroanatomy 2001-03, Vol.21 (2), p.149-159
Main Authors: Polkowska, Jolanta, Gładysz, Anna
Format: Article
Language:English
Subjects:
Animals
Body Weight - physiology
Diencephalon - anatomy & histology
Diencephalon - cytology
Diencephalon - metabolism
Diet
Dietary Proteins - pharmacology
Feeding
Female
Hypothalamus
Hypothalamus - anatomy & histology
Hypothalamus - drug effects
Hypothalamus - metabolism
Image Processing, Computer-Assisted
Immunohistochemistry
Neurons - metabolism
Neuropeptide Y - biosynthesis
Peptide
Sheep
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Summary:The expression of neuropeptide Y (NPY), which is involved in the neuromodulatory function associated with animal nutrition, growth and reproduction, depends on the nutritional status. However, the roles of individual components of food are not fully recognised. In this study, we evaluated the effects of dietary protein levels on the diencephalic population of NPY neurones. Female lambs were fed two diets equilibrated energetically but containing 8% protein in restricted diet or 18% protein in elevated diet, for 15 weeks starting with 6 months of age, during the first breeding period. Then, brain tissues were collected, fixed and used for the immunohistochemical localisation of NPY. Detection of NPY in diencephalon sections was followed by the image analysis and expressed as the percent area stained and optical density of immunostaining. Two distinct populations of the immunoreactive NPY perikarya were found, one in the infundibular nucleus; and the other in areas of diencephalon adjacent to the rostral hypothalamus. Long-term feeding the protein restricted diet caused a prominent expression of the immunoreactive material solely in the hypothalamic NPY neurones, particularly in those located in the entire periventricular area and in the infundibular nucleus. Both, percent area exhibiting positive staining and the density of immunoreactive NPY measured in the periventricular subdivision of the paraventricular nucleus were significantly higher ( P
ISSN:0891-0618
1873-6300
DOI:10.1016/S0891-0618(01)00089-8