Analysis of Bordetella pertussis isolates collected in Japan before and after introduction of acellular pertussis vaccines

Because of recent concern that whole-cell pertussis vaccination can drive antigenic divergence of circulating isolates of Bordetella pertussis, we compared 12 clinical isolates of B. pertussis collected in Japan, the first country to introduce acellular pertussis vaccines, with the vaccine strain. W...

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Bibliographic Details
Published in:Vaccine 2001-04, Vol.19 (23-24), p.3248-3252
Main Authors: Guiso, Nicole, Boursaux-Eude, Caroline, Weber, Christian, Hausman, Sally Z., Sato, Hiroko, Iwaki, Masaaki, Kamachi, Kazunari, Konda, Toshifumi, Burns, Drusilla L.
Format: Article
Language:English
Subjects:
Acellular vaccines
Antigenic Variation
Antigens, Bacterial - genetics
Antigens, Bacterial - isolation & purification
Bacteriology
Biological and medical sciences
Bordetella pertussis
Bordetella pertussis - genetics
Bordetella pertussis - immunology
Bordetella pertussis - isolation & purification
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
Electrophoresis, Gel, Pulsed-Field
Europe
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Humans
Japan
Microbiology
Pertussis Vaccine - immunology
Pertussis Vaccine - pharmacology
prn gene
ptx gene
Selection, Genetic
Vaccines, Acellular - immunology
Vaccines, Acellular - pharmacology
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
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Summary:Because of recent concern that whole-cell pertussis vaccination can drive antigenic divergence of circulating isolates of Bordetella pertussis, we compared 12 clinical isolates of B. pertussis collected in Japan, the first country to introduce acellular pertussis vaccines, with the vaccine strain. We used pulsed-field gel electrophoresis, sequencing of ptx and prn genes and expression of fimbriae. Most of the isolates collected before or after introduction of acellular vaccine possess similar restriction patterns. They contain ptx genes and prn alleles similar to the vaccine strain and to European isolates collected before the introduction of vaccination. Two recently collected isolates exhibiting a different pulsed-field gel electrophoresis pattern possess ptxS1 and prn alleles similar to the alleles harbored by European isolates circulating currently. Our preliminary results suggest that, if acellular pertussis vaccine-induced antigenic divergence exists, it is likely to be a slow or rare process.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(01)00013-5