The kinetics of hypoxanthine efflux from the rat brain

The brain efflux of radiolabelled hypoxanthine in the rat was rapid in the first minute after injection [ K eff(i)=0.21±0.06 min −1], which was saturable with a V max=13.08±0.81 nM min −1 g −1, and a high K m,app (67.2±13.4 μM); the K i,app for inosine was 31.5±7.6 μM. Capillary depletion analysis i...

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Published in:Brain research 2001-04, Vol.899 (1), p.248-250
Main Authors: Redzic, Zoran B., Isakovic, Aleksandra, Segal, Malcolm B., Thomas, Sarah A., Rakic, Ljubisa M.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biological Transport - physiology
Blood–brain barrier
Brain - metabolism
Brain efflux
Brain efflux index
Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges
Fundamental and applied biological sciences. Psychology
Hypoxanthine
Hypoxanthine - pharmacokinetics
Injections, Intraventricular
Kinetics
Nucleobase
purines
pyrimidines
Rats
Rats, Wistar
Vertebrates: nervous system and sense organs
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Summary:The brain efflux of radiolabelled hypoxanthine in the rat was rapid in the first minute after injection [ K eff(i)=0.21±0.06 min −1], which was saturable with a V max=13.08±0.81 nM min −1 g −1, and a high K m,app (67.2±13.4 μM); the K i,app for inosine was 31.5±7.6 μM. Capillary depletion analysis indicated that hypoxanthine accumulates in neurons and glia with the time. From cross-inhibition studies with different purines and pyrimidines, it suggests that these molecules could also be important substrates for this carrier.
ISSN:0006-8993
1872-6240
DOI:10.1016/S0006-8993(01)02115-1