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The kinetics of hypoxanthine efflux from the rat brain
The brain efflux of radiolabelled hypoxanthine in the rat was rapid in the first minute after injection [ K eff(i)=0.21±0.06 min −1], which was saturable with a V max=13.08±0.81 nM min −1 g −1, and a high K m,app (67.2±13.4 μM); the K i,app for inosine was 31.5±7.6 μM. Capillary depletion analysis i...
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| Published in: | Brain research 2001-04, Vol.899 (1), p.248-250 |
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| cites | cdi_FETCH-LOGICAL-c422t-f3f494ef49653523272e1fcd14f73197c3d9d07f35a9f65c17153a63ec98d2093 |
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| container_start_page | 248 |
| container_title | Brain research |
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| creator | Redzic, Zoran B. Isakovic, Aleksandra Segal, Malcolm B. Thomas, Sarah A. Rakic, Ljubisa M. |
| description | The brain efflux of radiolabelled hypoxanthine in the rat was rapid in the first minute after injection [
K
eff(i)=0.21±0.06 min
−1], which was saturable with a
V
max=13.08±0.81 nM min
−1 g
−1, and a high
K
m,app (67.2±13.4 μM); the
K
i,app for inosine was 31.5±7.6 μM. Capillary depletion analysis indicated that hypoxanthine accumulates in neurons and glia with the time. From cross-inhibition studies with different purines and pyrimidines, it suggests that these molecules could also be important substrates for this carrier. |
| doi_str_mv | 10.1016/S0006-8993(01)02115-1 |
| format | article |
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K
eff(i)=0.21±0.06 min
−1], which was saturable with a
V
max=13.08±0.81 nM min
−1 g
−1, and a high
K
m,app (67.2±13.4 μM); the
K
i,app for inosine was 31.5±7.6 μM. Capillary depletion analysis indicated that hypoxanthine accumulates in neurons and glia with the time. From cross-inhibition studies with different purines and pyrimidines, it suggests that these molecules could also be important substrates for this carrier.</description><identifier>ISSN: 0006-8993</identifier><identifier>EISSN: 1872-6240</identifier><identifier>DOI: 10.1016/S0006-8993(01)02115-1</identifier><identifier>PMID: 11311886</identifier><identifier>CODEN: BRREAP</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Biological Transport - physiology ; Blood–brain barrier ; Brain - metabolism ; Brain efflux ; Brain efflux index ; Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges ; Fundamental and applied biological sciences. Psychology ; Hypoxanthine ; Hypoxanthine - pharmacokinetics ; Injections, Intraventricular ; Kinetics ; Nucleobase ; purines ; pyrimidines ; Rats ; Rats, Wistar ; Vertebrates: nervous system and sense organs</subject><ispartof>Brain research, 2001-04, Vol.899 (1), p.248-250</ispartof><rights>2001 Elsevier Science B.V.</rights><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-f3f494ef49653523272e1fcd14f73197c3d9d07f35a9f65c17153a63ec98d2093</citedby><cites>FETCH-LOGICAL-c422t-f3f494ef49653523272e1fcd14f73197c3d9d07f35a9f65c17153a63ec98d2093</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14061070$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11311886$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Redzic, Zoran B.</creatorcontrib><creatorcontrib>Isakovic, Aleksandra</creatorcontrib><creatorcontrib>Segal, Malcolm B.</creatorcontrib><creatorcontrib>Thomas, Sarah A.</creatorcontrib><creatorcontrib>Rakic, Ljubisa M.</creatorcontrib><title>The kinetics of hypoxanthine efflux from the rat brain</title><title>Brain research</title><addtitle>Brain Res</addtitle><description>The brain efflux of radiolabelled hypoxanthine in the rat was rapid in the first minute after injection [
K
eff(i)=0.21±0.06 min
−1], which was saturable with a
V
max=13.08±0.81 nM min
−1 g
−1, and a high
K
m,app (67.2±13.4 μM); the
K
i,app for inosine was 31.5±7.6 μM. Capillary depletion analysis indicated that hypoxanthine accumulates in neurons and glia with the time. From cross-inhibition studies with different purines and pyrimidines, it suggests that these molecules could also be important substrates for this carrier.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Biological Transport - physiology</subject><subject>Blood–brain barrier</subject><subject>Brain - metabolism</subject><subject>Brain efflux</subject><subject>Brain efflux index</subject><subject>Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hypoxanthine</subject><subject>Hypoxanthine - pharmacokinetics</subject><subject>Injections, Intraventricular</subject><subject>Kinetics</subject><subject>Nucleobase</subject><subject>purines</subject><subject>pyrimidines</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0006-8993</issn><issn>1872-6240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNqFkEtPGzEQgC1UBAH6E1rtpRUcFmbstb0-oSriJSFxID1bjnesuN3spvYGhX_PkkTlyGVGM_rmoY-xbwiXCKiungFAlbUx4hzwAjiiLPGATbDWvFS8gi9s8h85Zic5_xlLIQwcsWNEgVjXasLUbEHF39jREH0u-lAsXlf9xnXDYuwVFEK73hQh9ctiGMHkhmKeXOzO2GFwbaav-3zKft_ezKb35ePT3cP012PpK86HMohQmYrGoKSQXHDNCYNvsApaoNFeNKYBHYR0JijpUaMUTgnypm44GHHKfu72rlL_b015sMuYPbWt66hfZ6s1aF4b_SmIupZaahhBuQN96nNOFOwqxaVLrxbBvpu1W7P2XZsFtFuzFse57_sD6_mSmo-pvcoR-LEHXPauDcl1PuYPrgKFsH3gesfR6O0lUrLZR-o8NTGRH2zTx09eeQOUqJLD</recordid><startdate>20010427</startdate><enddate>20010427</enddate><creator>Redzic, Zoran B.</creator><creator>Isakovic, Aleksandra</creator><creator>Segal, Malcolm B.</creator><creator>Thomas, Sarah A.</creator><creator>Rakic, Ljubisa M.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20010427</creationdate><title>The kinetics of hypoxanthine efflux from the rat brain</title><author>Redzic, Zoran B. ; Isakovic, Aleksandra ; Segal, Malcolm B. ; Thomas, Sarah A. ; Rakic, Ljubisa M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-f3f494ef49653523272e1fcd14f73197c3d9d07f35a9f65c17153a63ec98d2093</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Biological Transport - physiology</topic><topic>Blood–brain barrier</topic><topic>Brain - metabolism</topic><topic>Brain efflux</topic><topic>Brain efflux index</topic><topic>Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hypoxanthine</topic><topic>Hypoxanthine - pharmacokinetics</topic><topic>Injections, Intraventricular</topic><topic>Kinetics</topic><topic>Nucleobase</topic><topic>purines</topic><topic>pyrimidines</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Redzic, Zoran B.</creatorcontrib><creatorcontrib>Isakovic, Aleksandra</creatorcontrib><creatorcontrib>Segal, Malcolm B.</creatorcontrib><creatorcontrib>Thomas, Sarah A.</creatorcontrib><creatorcontrib>Rakic, Ljubisa M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Redzic, Zoran B.</au><au>Isakovic, Aleksandra</au><au>Segal, Malcolm B.</au><au>Thomas, Sarah A.</au><au>Rakic, Ljubisa M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The kinetics of hypoxanthine efflux from the rat brain</atitle><jtitle>Brain research</jtitle><addtitle>Brain Res</addtitle><date>2001-04-27</date><risdate>2001</risdate><volume>899</volume><issue>1</issue><spage>248</spage><epage>250</epage><pages>248-250</pages><issn>0006-8993</issn><eissn>1872-6240</eissn><coden>BRREAP</coden><abstract>The brain efflux of radiolabelled hypoxanthine in the rat was rapid in the first minute after injection [
K
eff(i)=0.21±0.06 min
−1], which was saturable with a
V
max=13.08±0.81 nM min
−1 g
−1, and a high
K
m,app (67.2±13.4 μM); the
K
i,app for inosine was 31.5±7.6 μM. Capillary depletion analysis indicated that hypoxanthine accumulates in neurons and glia with the time. From cross-inhibition studies with different purines and pyrimidines, it suggests that these molecules could also be important substrates for this carrier.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>11311886</pmid><doi>10.1016/S0006-8993(01)02115-1</doi><tpages>3</tpages></addata></record> |
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| ispartof | Brain research, 2001-04, Vol.899 (1), p.248-250 |
| issn | 0006-8993 1872-6240 |
| language | eng |
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| source | ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Biological Transport - physiology Blood–brain barrier Brain - metabolism Brain efflux Brain efflux index Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges Fundamental and applied biological sciences. Psychology Hypoxanthine Hypoxanthine - pharmacokinetics Injections, Intraventricular Kinetics Nucleobase purines pyrimidines Rats Rats, Wistar Vertebrates: nervous system and sense organs |
| title | The kinetics of hypoxanthine efflux from the rat brain |
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