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Reporter cell lines are useful tools for monitoring biological activity of nuclear receptor ligands
To characterize the specificity of synthetic compounds for nuclear receptors, we established stable cell lines expressing the luciferase gene and different wild‐type or chimaeric receptors. MCF‐7 cells, which express the oestrogen receptor α (ERα), and HeLa cells, which do not express the oestrogen...
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Published in: | Luminescence (Chichester, England) England), 2001-03, Vol.16 (2), p.153-158 |
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container_title | Luminescence (Chichester, England) |
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creator | Balaguer, Patrick Boussioux, Anne-Marie Demirpence, Ediz Nicolas, Jean-Claude |
description | To characterize the specificity of synthetic compounds for nuclear receptors, we established stable cell lines expressing the luciferase gene and different wild‐type or chimaeric receptors. MCF‐7 cells, which express the oestrogen receptor α (ERα), and HeLa cells, which do not express the oestrogen receptor, were transfected with a plasmid containing the luciferase gene downstream from a minimum promoter (β‐globin) and an oestrogen‐responsive element, generating the MELN and the HELN cell lines, respectively. MELN cells enabled the detection of compounds that bind to the ERα or interfere with its pathway. HELN cells were used to establish stable transfectants expressing different nuclear receptors containing the DNA‐binding domain of the oestrogen receptors. We thus established ERα or ERβ reporter cell lines by transfecting ERα or ERβ expression plasmids, and also retinoic acid receptor α, β or γ reporter cell lines by transfecting the chimaeric RAR gene, in which the DNA‐binding domain was replaced by the ERα DNA‐binding domain. Copyright © 2001 John Wiley & Sons, Ltd. |
doi_str_mv | 10.1002/bio.630 |
format | article |
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Copyright © 2001 John Wiley & Sons, Ltd.</description><identifier>ISSN: 1522-7235</identifier><identifier>EISSN: 1522-7243</identifier><identifier>DOI: 10.1002/bio.630</identifier><identifier>PMID: 11312541</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Cell Line ; chimaeric nuclear receptor ; Estrogens - metabolism ; firefly luciferase ; Genes, Reporter ; Humans ; Ligands ; Luciferases - genetics ; nuclear receptors ligands ; Receptor Cross-Talk ; Receptors, Cytoplasmic and Nuclear - metabolism ; Retinoids - metabolism ; stable transfection ; subtype-selective retinoids ; xeno-oestrogens</subject><ispartof>Luminescence (Chichester, England), 2001-03, Vol.16 (2), p.153-158</ispartof><rights>Copyright © 2001 John Wiley & Sons, Ltd.</rights><rights>Copyright 2001 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3510-307e9fc4408018c7568214bb18e795061c5ba3c7360e9783b9e5c77d181bd0673</citedby><cites>FETCH-LOGICAL-c3510-307e9fc4408018c7568214bb18e795061c5ba3c7360e9783b9e5c77d181bd0673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11312541$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Balaguer, Patrick</creatorcontrib><creatorcontrib>Boussioux, Anne-Marie</creatorcontrib><creatorcontrib>Demirpence, Ediz</creatorcontrib><creatorcontrib>Nicolas, Jean-Claude</creatorcontrib><title>Reporter cell lines are useful tools for monitoring biological activity of nuclear receptor ligands</title><title>Luminescence (Chichester, England)</title><addtitle>Luminescence</addtitle><description>To characterize the specificity of synthetic compounds for nuclear receptors, we established stable cell lines expressing the luciferase gene and different wild‐type or chimaeric receptors. 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Copyright © 2001 John Wiley & Sons, Ltd.</description><subject>Cell Line</subject><subject>chimaeric nuclear receptor</subject><subject>Estrogens - metabolism</subject><subject>firefly luciferase</subject><subject>Genes, Reporter</subject><subject>Humans</subject><subject>Ligands</subject><subject>Luciferases - genetics</subject><subject>nuclear receptors ligands</subject><subject>Receptor Cross-Talk</subject><subject>Receptors, Cytoplasmic and Nuclear - metabolism</subject><subject>Retinoids - metabolism</subject><subject>stable transfection</subject><subject>subtype-selective retinoids</subject><subject>xeno-oestrogens</subject><issn>1522-7235</issn><issn>1522-7243</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNp1kE1v1DAQhi0EYsuC-AfIJzigbO04tpMjXbVL0ZaKio-j5TiTlcGJg53Q7r-vV1ltT5xmDo-emfdF6C0lK0pIfl5bvxKMPENnlOd5JvOCPT_tjC_Qqxh_E0KEENVLtKCU0ZwX9AyZOxh8GCFgA85hZ3uIWAfAU4R2cnj03kXc-oA739vRB9vvcLrm_M4a7bA2o_1nxz32Le4n40AHHMDAkNBk2-m-ia_Ri1a7CG-Oc4l-XF1-X3_Otreb6_WnbWYYpyRjRELVmqIgJaGlkVyUOS3qmpYgK04ENbzWzEgmCFSyZHUF3EjZ0JLWDRGSLdH72TsE_3eCOKrOxkMs3YOfopKSSFbIKoEfZtAEH2OAVg3BdjrsFSXq0KdKCVXqM5Hvjsqp7qB54o4FJuDjDNxbB_v_edTF9e2sy2baxhEeTrQOf1T6X3L16-tG3fwU-eZu_UV9Y4_UNo16</recordid><startdate>200103</startdate><enddate>200103</enddate><creator>Balaguer, Patrick</creator><creator>Boussioux, Anne-Marie</creator><creator>Demirpence, Ediz</creator><creator>Nicolas, Jean-Claude</creator><general>John Wiley & Sons, Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200103</creationdate><title>Reporter cell lines are useful tools for monitoring biological activity of nuclear receptor ligands</title><author>Balaguer, Patrick ; Boussioux, Anne-Marie ; Demirpence, Ediz ; Nicolas, Jean-Claude</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3510-307e9fc4408018c7568214bb18e795061c5ba3c7360e9783b9e5c77d181bd0673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Cell Line</topic><topic>chimaeric nuclear receptor</topic><topic>Estrogens - metabolism</topic><topic>firefly luciferase</topic><topic>Genes, Reporter</topic><topic>Humans</topic><topic>Ligands</topic><topic>Luciferases - genetics</topic><topic>nuclear receptors ligands</topic><topic>Receptor Cross-Talk</topic><topic>Receptors, Cytoplasmic and Nuclear - metabolism</topic><topic>Retinoids - metabolism</topic><topic>stable transfection</topic><topic>subtype-selective retinoids</topic><topic>xeno-oestrogens</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Balaguer, Patrick</creatorcontrib><creatorcontrib>Boussioux, Anne-Marie</creatorcontrib><creatorcontrib>Demirpence, Ediz</creatorcontrib><creatorcontrib>Nicolas, Jean-Claude</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Luminescence (Chichester, England)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Balaguer, Patrick</au><au>Boussioux, Anne-Marie</au><au>Demirpence, Ediz</au><au>Nicolas, Jean-Claude</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Reporter cell lines are useful tools for monitoring biological activity of nuclear receptor ligands</atitle><jtitle>Luminescence (Chichester, England)</jtitle><addtitle>Luminescence</addtitle><date>2001-03</date><risdate>2001</risdate><volume>16</volume><issue>2</issue><spage>153</spage><epage>158</epage><pages>153-158</pages><issn>1522-7235</issn><eissn>1522-7243</eissn><abstract>To characterize the specificity of synthetic compounds for nuclear receptors, we established stable cell lines expressing the luciferase gene and different wild‐type or chimaeric receptors. MCF‐7 cells, which express the oestrogen receptor α (ERα), and HeLa cells, which do not express the oestrogen receptor, were transfected with a plasmid containing the luciferase gene downstream from a minimum promoter (β‐globin) and an oestrogen‐responsive element, generating the MELN and the HELN cell lines, respectively. MELN cells enabled the detection of compounds that bind to the ERα or interfere with its pathway. HELN cells were used to establish stable transfectants expressing different nuclear receptors containing the DNA‐binding domain of the oestrogen receptors. We thus established ERα or ERβ reporter cell lines by transfecting ERα or ERβ expression plasmids, and also retinoic acid receptor α, β or γ reporter cell lines by transfecting the chimaeric RAR gene, in which the DNA‐binding domain was replaced by the ERα DNA‐binding domain. Copyright © 2001 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>11312541</pmid><doi>10.1002/bio.630</doi><tpages>6</tpages></addata></record> |
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subjects | Cell Line chimaeric nuclear receptor Estrogens - metabolism firefly luciferase Genes, Reporter Humans Ligands Luciferases - genetics nuclear receptors ligands Receptor Cross-Talk Receptors, Cytoplasmic and Nuclear - metabolism Retinoids - metabolism stable transfection subtype-selective retinoids xeno-oestrogens |
title | Reporter cell lines are useful tools for monitoring biological activity of nuclear receptor ligands |
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