Contribution of MHC class I-dependent immune mechanisms induced by attenuated recombinant Salmonella typhimurium secreting superoxide dismutase to protection against murine listeriosis

• Published in: Vaccine 2001-04, Vol.19 (23-24), p.3269-3272
• Main Authors: Grode, Leander, Kaufmann, Stefan H.E., Hess, Jürgen
• Format: Article
• Language: English
• Subjects: Animals; ATP Binding Cassette Transporter, Subfamily B, Member 2; ATP-Binding Cassette Transporters - genetics; Bacterial Vaccines - pharmacology; Bacteriology; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; histocompatibility antigen H-2; Histocompatibility Antigens Class I - metabolism; Immune mechanism; Immunity, Cellular; Listeria monocytogenes - enzymology; Listeria monocytogenes - genetics; Listeria monocytogenes - immunology; Listeriosis - immunology; Listeriosis - prevention & control; Mice; Mice, Inbred C57BL; Mice, Knockout; Microbiology; Murine listeriosis; Recombination, Genetic; Salmonella typhimurium; Salmonella typhimurium - genetics; Salmonella typhimurium - immunology; Superoxide dismutase; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccines, Attenuated - pharmacology
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/S0264-410X(01)00022-6

A recombinant (r)Salmonella typhimuriumaroA strain secreting the naturally non-secreted superoxide dismutase (SOD) of Listeria monocytogenes controls murine listeriosis dependent on ‘transporter associated with antigen processing’ (TAP)-mediated immune mechanisms. TAP1-deficient mice (devoid of most CD8 T cells) vaccinated with this rSalmonella SODs strain succumbed to lethal L. monocytogenes challenge, whereas C57BL/6 mice were protected by this vaccine. Moreover, vaccination of H-2I-Aβ-deficient mice (lacking major histocompatibility class (MHC) II molecules and thus devoid of mature CD4 TCR-αβ cells), of TAP1-deficient as well as of β2microglobulin-deficient mice (devoid of conventional CD8 T cells) with a sublethal dose of L. monocytogenes and subsequent challenge with rSalmonella control or SODs strain revealed contribution of both MHC class I- and MHC class II-dependent immune mechanisms to the control of secondary Salmonella infection. Finally, the clearance of rSalmonella SODs bacteria was achieved in TAP1-deficient animals vaccinated with L. monocytogenes. Our data suggest a role of TAP-dependent mechanisms in priming of protective immunity by rSalmonella micro-organisms secreting SOD.